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Related Experiment Video

Updated: Jun 16, 2026

Direct Induction of Human Neural Stem Cells from Peripheral Blood Hematopoietic Progenitor Cells
12:06

Direct Induction of Human Neural Stem Cells from Peripheral Blood Hematopoietic Progenitor Cells

Published on: January 28, 2015

[Peripheral blood hematopoietic stem cell collection].

Ines Bojanić1, Sanja Mazić, Branka Golubić Cepulić

  • 1Zavod za transfuzijsku medicinu i stanicnu terapiju, Klinicki bolnicki centar Zagreb, Kispatićeva 12, 10000 Zagreb. ibojanic@kbc-zagreb.hr

Lijecnicki Vjesnik
|February 11, 2010
PubMed
Summary

Peripheral blood hematopoietic stem cells (PBSC) offer advantages over bone marrow transplants, with leukapheresis being a simpler collection method. Advanced techniques like large-volume leukapheresis improve stem cell yield for "poor mobilizers".

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Area of Science:

  • Hematology
  • Transplantation Immunology
  • Cellular Therapy

Context:

  • Peripheral blood hematopoietic stem cells (PBSC) are increasingly preferred over traditional bone marrow for transplantation.
  • Leukapheresis is a less invasive and better-tolerated method for PBSC collection compared to bone marrow harvest.
  • PBSC mobilization involves myelosuppressive chemotherapy and/or hematopoietic growth factors, enhancing stem cell yield.

Purpose:

  • To compare the advantages of PBSC collection via leukapheresis with bone marrow harvest.
  • To discuss strategies for optimizing PBSC collection in patients with poor mobilization responses.
  • To outline the composition of leukapheresis products and their role in hematopoietic recovery.

Summary:

  • Leukapheresis products contain PBSC, committed progenitors, and precursors, facilitating faster hematopoietic recovery.

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Isolation and Transplantation of Hematopoietic Stem Cells (HSCs)
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Isolation and Transplantation of Hematopoietic Stem Cells (HSCs)

Published on: February 25, 2007

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Last Updated: Jun 16, 2026

Direct Induction of Human Neural Stem Cells from Peripheral Blood Hematopoietic Progenitor Cells
12:06

Direct Induction of Human Neural Stem Cells from Peripheral Blood Hematopoietic Progenitor Cells

Published on: January 28, 2015

Isolation and Transplantation of Hematopoietic Stem Cells (HSCs)
20:38

Isolation and Transplantation of Hematopoietic Stem Cells (HSCs)

Published on: February 25, 2007

  • For "poor mobilizers," large-volume leukapheresis (LVL) or novel agents like pegfilgrastim and CXCR4 antagonists are effective.
  • LVL processes a larger blood volume (more than 3x) compared to standard leukapheresis (2-3x), resulting in significantly higher CD34+ cell counts.
  • Potential adverse effects of leukapheresis include hypocalcemia from citrate and bleeding risks due to thrombocytopenia and heparin administration.
  • PBSC collection and product quality are governed by national and international standards.

    • Impact:

    • PBSC transplantation offers a more efficient and patient-friendly alternative to bone marrow transplantation.
    • Improved mobilization strategies and collection techniques enhance the success rates of stem cell therapies.
    • Standardized quality control ensures the safety and efficacy of PBSC products for clinical use.