Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Video

Updated: Jun 16, 2026

Synovial Fluid Analysis to Identify Osteoarthritis
07:51

Synovial Fluid Analysis to Identify Osteoarthritis

Published on: October 20, 2022

Alpha-mannosidase activity in synovial fluid.

B A Bartholomew1, A L Perry

  • 1Dept. of Internal Medicine, Section of Rheumatic Diseases, University of Colorado Medical Center, Denver, Colorado, USA.

Scandinavian Journal of Rheumatology
|February 11, 2010
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

Glycosaminoglycans01:23

Glycosaminoglycans

Glycosaminoglycans (GAGs), also known as mucopolysaccharides, are long and linear polymers comprising of specific repeating disaccharides - the amino sugar that can be N-acetylglucosamine or N-acetylgalactosamine, and a uronic acid that is usually glucuronic acid or iduronic acid.
GAGS are found in the extracellular matrix of vertebrates, invertebrates, and bacteria. Due to their polar nature they attract water, and serve as excellent lubricants or shock absorbers in an animal body.
Hyaluronic...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Remote chemical immobilisation method for free-ranging Australian cattle.

Australian veterinary journal·2016
Same author

Vertebral osteomyelitis and multiple cutaneous lesions in a dog caused by Nocardia pseudobrasiliensis.

Journal of veterinary internal medicine·2014
Same author

Characterization of HOX gene expression in canine mammary tumour cell lines from spontaneous tumours.

Veterinary and comparative oncology·2013
Same author

Comparison of five commercial anti-tetanus toxoid immunoglobulin G enzyme-linked immunosorbent assays.

Clinical and vaccine immunology : CVI·2009
Same author

Propionibacterium acnes.

Letters in applied microbiology·2006
Same author

Enzymology of oxidation of tropic acid to phenylacetic acid in metabolism of atropine by Pseudomonas sp. strain AT3.

Journal of bacteriology·1997
Same journal

Sámi psoriatic arthritis patients in northern Norway receive less conventional synthetic disease-modifying anti-rheumatic drug treatment and have more arthritis, axial symptoms, and joint damage.

Scandinavian journal of rheumatology·2026
Same journal

Distinct clinical outcomes of positron emission tomography/computed tomography-based subtypes in treatment-naïve Takayasu arteritis.

Scandinavian journal of rheumatology·2026
Same journal

Elevated serum immunoglobulin A predicts extra-articular manifestations in axial spondyloarthritis.

Scandinavian journal of rheumatology·2026
Same journal

Multimodal imaging in the diagnosis of iliopsoas bursitis complicating biological therapy in rheumatoid arthritis: a case report with therapeutic implications.

Scandinavian journal of rheumatology·2026
Same journal

Real-world clinical outcomes of patients with disproportionate articular pain in rheumatoid arthritis: ANSWER cohort study.

Scandinavian journal of rheumatology·2026
Same journal

Cervical spine instability in axial spondyloarthritis: prevalence, patterns, and predictive factors in a high-risk cohort.

Scandinavian journal of rheumatology·2026
See all related articles

A new microassay accurately measures alpha-mannosidase in synovial fluid. Enzyme levels correlate with inflammation and cell counts in joint disease, offering a quantitative diagnostic tool.

Area of Science:

  • Biochemistry
  • Rheumatology
  • Enzymology

Background:

  • Synovial fluid analysis is crucial for diagnosing joint diseases.
  • Quantifying inflammatory markers in synovial fluid aids in disease assessment.
  • Lysosomal glycosidases, like alpha-mannosidase, are implicated in inflammatory processes.

Purpose of the Study:

  • To develop and validate a microassay for synovial fluid alpha-mannosidase activity.
  • To investigate the correlation between alpha-mannosidase activity and indicators of joint inflammation.
  • To explore the potential of alpha-mannosidase as a biomarker for arthropathic conditions.

Main Methods:

  • Development of a microassay technique for alpha-mannosidase activity measurement.
  • Analysis of 184 synovial fluid samples from patients with diverse arthropathic conditions.

More Related Videos

A Friction Testing-Bioreactor Device for Study of Synovial Joint Biomechanics, Mechanobiology, and Physical Regulation
09:48

A Friction Testing-Bioreactor Device for Study of Synovial Joint Biomechanics, Mechanobiology, and Physical Regulation

Published on: June 2, 2022

Related Experiment Videos

Last Updated: Jun 16, 2026

Synovial Fluid Analysis to Identify Osteoarthritis
07:51

Synovial Fluid Analysis to Identify Osteoarthritis

Published on: October 20, 2022

A Friction Testing-Bioreactor Device for Study of Synovial Joint Biomechanics, Mechanobiology, and Physical Regulation
09:48

A Friction Testing-Bioreactor Device for Study of Synovial Joint Biomechanics, Mechanobiology, and Physical Regulation

Published on: June 2, 2022

  • Correlation analysis between enzyme activity, synovial fluid cell count, and inflammation markers.

    • Main Results:

    • The microassay demonstrated reliable measurement of synovial fluid alpha-mannosidase.
    • A significant correlation was observed between enzyme activity and synovial fluid cell count.
    • Enzyme activity closely correlated with the degree of inflammation in various joint diseases.

    Conclusions:

    • Synovial fluid alpha-mannosidase activity can be reliably quantified using the developed microassay.
    • Alpha-mannosidase activity serves as a quantitative indicator of inflammation in joint diseases.
    • Evaluation of alpha-mannosidase and other lysosomal glycosidases offers a valuable method for assessing inflammatory joint disease.