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Related Concept Videos

Autophagy01:27

Autophagy

Autophagy is a self-digesting process by which a cell protects itself from threats both within and outside the cell, ranging from abnormal proteins to invading bacteria. In this process, obsolete components of the cell and invading microbes are degraded by hydrolytic enzymes active in an acidic environment of the lysosomal lumen.
An autophagic pathway consists of a series of signaling events activated in response to diverse stress and physiological conditions such as food deprivation,...
Delivery Pathways to the Lysosome01:36

Delivery Pathways to the Lysosome

Eukaryotic cells use different mechanisms to eliminate toxic waste obsolete and worn-out substances. Lysosomes play a pivotal role in this, and hence, these substances are carried to the lysosome from other parts of the cell and extracellular space through different pathways. The most elaborately studied pathways to the lysosome are the endocytic pathways.
Endocytosis
In endocytosis, the cell membrane takes up macromolecules and particles from the surrounding medium. Clathrin-mediated...
Autophagic Cell Death01:18

Autophagic Cell Death

Christian de Duve discovered “autophagy,” a process in which cellular components are engulfed by membrane-bound organelles called autophagosomes. The autophagosomes then fuse with lysosomes to digest the enclosed contents. Autophagy is generally activated in cells to prevent cell death. However, cell death is triggered when the damage is beyond repair.
Autophagy and Apoptosis
Autophagy can activate apoptosis. In normal conditions, the autophagy activating protein Beclin-1 and pro-apoptotic...

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Related Experiment Video

Updated: Jun 16, 2026

The Lactate Dehydrogenase Sequestration Assay &#8212; A Simple and Reliable Method to Determine Bulk Autophagic Sequestration Activity in Mammalian Cells
09:34

The Lactate Dehydrogenase Sequestration Assay — A Simple and Reliable Method to Determine Bulk Autophagic Sequestration Activity in Mammalian Cells

Published on: July 27, 2018

Methods in mammalian autophagy research.

Noboru Mizushima1, Tamotsu Yoshimori, Beth Levine

  • 1Department of Physiology and Cell Biology, Tokyo Medical and Dental University, Tokyo 113-8519, Japan. nmizu.phy2@tmd.ac.jp

Cell
|February 11, 2010
PubMed
Summary
This summary is machine-generated.

This primer explains how to accurately monitor and modulate autophagy, a vital cellular process. Understanding these methods is crucial for research in many physiological and pathological conditions.

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Assessing Autophagic Flux by Measuring LC3, p62, and LAMP1 Co-localization Using Multispectral Imaging Flow Cytometry
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Assessing Autophagic Flux by Measuring LC3, p62, and LAMP1 Co-localization Using Multispectral Imaging Flow Cytometry

Published on: July 21, 2017

Live Cell Imaging of Early Autophagy Events: Omegasomes and Beyond
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Live Cell Imaging of Early Autophagy Events: Omegasomes and Beyond

Published on: July 27, 2013

Related Experiment Videos

Last Updated: Jun 16, 2026

The Lactate Dehydrogenase Sequestration Assay &#8212; A Simple and Reliable Method to Determine Bulk Autophagic Sequestration Activity in Mammalian Cells
09:34

The Lactate Dehydrogenase Sequestration Assay — A Simple and Reliable Method to Determine Bulk Autophagic Sequestration Activity in Mammalian Cells

Published on: July 27, 2018

Assessing Autophagic Flux by Measuring LC3, p62, and LAMP1 Co-localization Using Multispectral Imaging Flow Cytometry
11:39

Assessing Autophagic Flux by Measuring LC3, p62, and LAMP1 Co-localization Using Multispectral Imaging Flow Cytometry

Published on: July 21, 2017

Live Cell Imaging of Early Autophagy Events: Omegasomes and Beyond
09:00

Live Cell Imaging of Early Autophagy Events: Omegasomes and Beyond

Published on: July 27, 2013

Area of Science:

  • Cell Biology
  • Molecular Biology
  • Physiology

Background:

  • Autophagy plays a critical role in numerous physiological and pathological processes.
  • Accurate identification, quantification, and manipulation of autophagy are essential for scientific advancement.
  • Current analytical methods for autophagy are often imprecise due to its complex and dynamic nature.

Purpose of the Study:

  • To provide a comprehensive guide on monitoring and modulating autophagy.
  • To address the challenges in accurately analyzing autophagic activity.
  • To focus on methods applicable to mammalian macroautophagy.

Main Methods:

  • Discussion of various techniques for monitoring autophagic flux.
  • Explanation of strategies for modulating autophagic activity.
  • Emphasis on practical considerations for analyzing mammalian macroautophagy.

Main Results:

  • Provides clarity on the complexities of autophagy analysis.
  • Highlights common pitfalls in current autophagy research.
  • Offers solutions for more accurate assessment of autophagic processes.

Conclusions:

  • Accurate monitoring and modulation of autophagy are achievable with appropriate methods.
  • This primer serves as a valuable resource for researchers studying autophagy.
  • Improved understanding and application of these techniques will advance autophagy research.