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Related Concept Videos

Chromatin Position Affects Gene Expression02:35

Chromatin Position Affects Gene Expression

Chromatin is the massive complex of DNA and proteins packaged inside the nucleus. The complexity of chromatin folding and how it is packaged inside the nucleus greatly influences  access to genetic information. Generally, the nucleus' periphery is considered transcriptionally repressive, while the cell's interior is considered a transcriptionally active area. 
Topologically Associated Domains (TADs)
The 3-dimensional positioning of chromatin in the nucleus influences the timing and level of...
Chromatin Structure Regulates pre-mRNA Processing02:41

Chromatin Structure Regulates pre-mRNA Processing

In eukaryotic cells, nascent mRNA transcripts need to undergo many post-transcriptional modifications to reach the cell cytoplasm and translate into functional proteins. For a long time, transcription and pre-mRNA processing were considered two independent events that occur sequentially in the cell. However, it has now been well established that transcription and pre-mRNA processing are two simultaneous processes that are precisely regulated inside the cell.
The chromatin structure, especially...
Regulation of Nuclear Protein Sorting01:45

Regulation of Nuclear Protein Sorting

Nuclear protein sorting regulates nucleus composition and gene expression, crucial for determining the fate of a eukaryotic cell. Hence, the entry and exit of molecules across the nuclear envelope is a tightly controlled process. Nuclear protein sorting can be inhibited by one of the following ways: 1) masking cargo signal sequences, 2) modifying the nuclear receptor's affinity for cargo, 3) controlling the nuclear pore size, 4) retaining the cargo during its transit to the cytosol or the...
Euchromatin01:01

Euchromatin

The extent of chromatin compaction can be studied by staining chromatin using specific DNA binding dyes. Under the microscope, the dense-compacted regions take up more dye, appearing darker, while the less-compact areas take up less dye and appear lighter. Based on the compaction level, chromatins are classified into two primary forms – euchromatin and heterochromatin.
Euchromatin is the less dense region of the chromatin and stains lighter. Euchromatin contains histone H3 extensively...
Euchromatin01:01

Euchromatin

The extent of chromatin compaction can be studied by staining chromatin using specific DNA binding dyes. Under the microscope, the dense-compacted regions take up more dye, appearing darker, while the less-compact areas take up less dye and appear lighter. Based on the compaction level, chromatins are classified into two primary forms – euchromatin and heterochromatin.
Euchromatin is the less dense region of the chromatin and stains lighter. Euchromatin contains histone H3 extensively...
Heterochromatin02:38

Heterochromatin

The extent of chromatin compaction can be studied by staining chromatin using specific DNA binding dyes. Under the microscope, the dense-compacted regions that take up more dye are called heterochromatin. Heterochromatin is further classified into two forms – constitutive heterochromatin and facultative heterochromatin.
Constitutive heterochromatin: It is a highly compact region of chromatin that is mostly concentrated in the centromere and telomere. Unlike euchromatin, the amino acid at 9th...

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Mapping Absolute DNA Density in Cell Nuclei using Single-molecule Localization Microscopy
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Published on: November 11, 2025

Chromatin-bound nuclear pore components regulate gene expression in higher eukaryotes.

Maya Capelson1, Yun Liang, Roberta Schulte

  • 1Molecular and Cellular Biology Laboratory, The Salk Institute for Biological Studies, 10010 North Torrey Pines Road, La Jolla, CA 92037, USA.

Cell
|February 11, 2010
PubMed
Summary
This summary is machine-generated.

Nuclear pore complex proteins Sec13 and Nup98 regulate gene expression in fruit flies. Knockdown inhibits gene transcription and reactivation, highlighting their role in multicellular gene programs.

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Last Updated: Jun 16, 2026

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Area of Science:

  • Molecular Biology
  • Genetics
  • Cell Biology

Background:

  • Nuclear pore complexes (NPCs) are known to influence gene activation.
  • The role of NPCs in metazoan transcription regulation is not fully understood.

Purpose of the Study:

  • To investigate the involvement of specific nucleoporins in metazoan gene regulation.
  • To determine the functional significance of NPC components in transcription.

Main Methods:

  • Genome-wide binding analysis of nucleoporins Sec13, Nup98, Nup88, and FG-repeat nucleoporins in Drosophila.
  • RNA interference (RNAi)-mediated knockdown of Sec13 and Nup98.
  • Assessment of gene transcription and reactivation after heat shock.

Main Results:

  • Sec13, Nup98, and FG-repeat nucleoporins bind to distinct genomic loci, often not at the nuclear envelope.
  • Nup88 localizes to silent loci, while Sec13, Nup98, and some FG-repeat nucleoporins associate with transcriptionally active genes.
  • Knockdown of intranuclear Sec13 and Nup98 inhibits target gene transcription and prevents reactivation after heat shock.

Conclusions:

  • Intranuclear nucleoporins Sec13 and Nup98 are essential for regulating gene expression programs in multicellular organisms.
  • NPC components play a critical role beyond nuclear transport, influencing complex gene expression dynamics.