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Pilot quality assurance programme for plasma metanephrines.

Dilo Pillai¹, Shaw Callen

¹Department of Clinical Chemistry, SEALS, The Prince of Wales Hospital, Randwick, Australia. dpillai@nsccahs.health.nsw.gov.au

Annals of Clinical Biochemistry

|February 11, 2010

Summary

A pilot study evaluated external quality assurance for plasma free metanephrines. Enzyme immunoassays showed higher imprecision and bias compared to chromatography, highlighting the need for standardization.

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Area of Science:

Clinical Biochemistry
Analytical Chemistry

Background:

No formal external quality assurance (EQA) program existed for plasma free metanephrines before 2007.
A pilot EQA program was developed by the Australian Association of Clinical Biochemists (AACB) Working Party on biogenic amines.

Purpose of the Study:

To assess the performance of different analytical methods for plasma free metanephrines.
To evaluate the feasibility of an external quality assurance program for these analytes.

Main Methods:

Distribution of 12 lyophilized plasma samples spiked with metanephrine, normetanephrine, and 3-methoxytyramine to 15 participating centers.
Samples contained analytes at six concentration levels, with duplicated samples, to assess precision and accuracy.

Main Results:

High-performance liquid chromatography (HPLC) and tandem mass spectrometry (MS/MS) methods demonstrated acceptable precision.
Enzyme immunoassays generally exhibited higher imprecision and a negative bias compared to chromatographic methods.

Conclusions:

Discrepancies between immunoassay and chromatographic methods are likely due to calibration differences and matrix effects.
Standardization efforts are hindered by the absence of a universal calibrator for plasma free metanephrines.
Participation in EQA programs can enhance laboratory performance monitoring and improvement.