Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Super-resolution Fluorescence Microscopy01:37

Super-resolution Fluorescence Microscopy

Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been developed.

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Normative volumetric growth modeling of the whole fetal body, placenta, and amniotic fluid for three-dimensional T2-weighted magnetic resonance imaging.

Pediatric radiology·2026
Same author

EACVI survey on the use of multi-modality cardiovascular imaging in immune-mediated inflammatory diseases.

European heart journal. Imaging methods and practice·2026
Same author

Four-dimensional left ventricular motion clustering reveals cardiovascular phenotypes at population scale.

Scientific reports·2026
Same author

Neonatal brain structure, cognitively stimulating parenting and behavioural outcomes in preschool children with congenital heart disease and controls.

Scientific reports·2026
Same author

Quantitative ventricular trabeculation assessment in cardiac MRI: optimised blood-pool segmentation, box-counting fractal analysis and non-fractal measurements.

The international journal of cardiovascular imaging·2026
Same author

Left Ventricular Noncompaction: Time to Retire a Flawed Diagnosis.

Journal of the American College of Cardiology·2026
Same journal

A Comparison of Tissue Property Values Estimated Using Conventional Cardiac MRF and MT-Cardiac MRF.

Magnetic resonance in medicine·2026
Same journal

Dependence of the Extra-Cellular Diffusion Coefficient on the Fractions of Neurites and Cell Bodies in Gray Matter.

Magnetic resonance in medicine·2026
Same journal

Triple-Pulse <sup>23</sup>Na MRI Sequence (TriNa) for Simultaneous Acquisition of Spin-Density-Weighted and Fluid-Attenuated Images.

Magnetic resonance in medicine·2026
Same journal

Evaluation of Phantom Doping Materials in Quantitative Susceptibility Mapping.

Magnetic resonance in medicine·2026
Same journal

Design of an 8-Channel Transmit 32-Channel Receive 11.7T Head Coil and Evaluation of SNR Gains.

Magnetic resonance in medicine·2026
Same journal

The Potential for Absolute Temperature Imaging Based on Brain Metabolites Using an FID-Shifting Approach in Gradient Echo Planar Spectroscopic Imaging (GREPSI).

Magnetic resonance in medicine·2026
See all related articles

Related Experiment Video

Updated: Jun 16, 2026

Biomolecular Imaging of Cellular Uptake of Nanoparticles using Multimodal Nonlinear Optical Microscopy
07:13

Biomolecular Imaging of Cellular Uptake of Nanoparticles using Multimodal Nonlinear Optical Microscopy

Published on: May 16, 2022

Subject-specific water-selective imaging using parallel transmission.

Shaihan J Malik1, David J Larkman, Declan P O'Regan

  • 1Robert Steiner MRI Unit, Imaging Sciences Department, Hammersmith Hospital Campus, Imperial College London, London, UK. shaihan.malik03@imperial.ac.uk

Magnetic Resonance in Medicine
|February 11, 2010
PubMed
Summary
This summary is machine-generated.

This study introduces subject-specific spectral-spatial pulses for MRI, improving fat suppression and reducing radiofrequency power. This method enhances imaging reliability by accounting for individual magnetic field variations.

More Related Videos

High-Throughput Total Internal Reflection Fluorescence and Direct Stochastic Optical Reconstruction Microscopy Using a Photonic Chip
14:09

High-Throughput Total Internal Reflection Fluorescence and Direct Stochastic Optical Reconstruction Microscopy Using a Photonic Chip

Published on: November 16, 2019

A Guide to Structured Illumination TIRF Microscopy at High Speed with Multiple Colors
11:15

A Guide to Structured Illumination TIRF Microscopy at High Speed with Multiple Colors

Published on: May 30, 2016

Related Experiment Videos

Last Updated: Jun 16, 2026

Biomolecular Imaging of Cellular Uptake of Nanoparticles using Multimodal Nonlinear Optical Microscopy
07:13

Biomolecular Imaging of Cellular Uptake of Nanoparticles using Multimodal Nonlinear Optical Microscopy

Published on: May 16, 2022

High-Throughput Total Internal Reflection Fluorescence and Direct Stochastic Optical Reconstruction Microscopy Using a Photonic Chip
14:09

High-Throughput Total Internal Reflection Fluorescence and Direct Stochastic Optical Reconstruction Microscopy Using a Photonic Chip

Published on: November 16, 2019

A Guide to Structured Illumination TIRF Microscopy at High Speed with Multiple Colors
11:15

A Guide to Structured Illumination TIRF Microscopy at High Speed with Multiple Colors

Published on: May 30, 2016

Area of Science:

  • Magnetic Resonance Imaging (MRI)
  • Pulse Sequence Design
  • Medical Physics

Background:

  • Spectral-spatial excitation pulses offer efficient water- or fat-only imaging.
  • Their reliability is limited by static magnetic field inhomogeneity.
  • Longer pulses are needed to mitigate field variations, impacting imaging time.

Purpose of the Study:

  • To develop a subject-specific optimization method for spectral-spatial pulses.
  • To improve the reliability and performance of fat suppression in MRI.
  • To reduce radiofrequency (RF) power deposition while maintaining imaging quality.

Main Methods:

  • Optimized spectral-spatial pulses on a subject-specific basis using measured field variations.
  • Employed multichannel RF transmission for precise control without increasing pulse duration.
  • Applied the method to abdominal imaging at 3 Tesla using an eight-channel system.

Main Results:

  • Subject-specific pulses significantly improved fat suppression in all five healthy volunteers.
  • Reduced RF power by 13% +/- 6%.
  • Demonstrated reduced mean flip angle in fat (0.12° +/- 0.04° vs. 0.72° +/- 0.55°) and improved water excitation uniformity (std/mean 0.16 +/- 0.05 vs. 0.26 +/- 0.05).

Conclusions:

  • Subject-specific optimization enhances spectral-spatial pulse performance in MRI.
  • The proposed method offers a reliable approach to fat suppression, reducing RF power and improving image uniformity.
  • This technique holds promise for more robust and efficient MRI applications.