Related Experiment Video

Updated: Jun 16, 2026

In ovo Electroporation of miRNA-based Plasmids in the Developing Neural Tube and Assessment of Phenotypes by DiI Injection in Open-book Preparations
12:41

In ovo Electroporation of miRNA-based Plasmids in the Developing Neural Tube and Assessment of Phenotypes by DiI Injection in Open-book Preparations

Published on: October 16, 2012

Choosing the sequence of dsRNA for RNAi in mouse

Petr Svoboda1

  • 1Institute of Molecular Genetics, Academy of Sciences of Czech Republic, Videnska 1083, 142 20 Prague 4, Czech Republic. svobodap@img.cas.cz

Cold Spring Harbor Protocols
|February 12, 2010
PubMed

Abstract:

RNA interference (RNAi) is a suitable method for sequence-specific post-transcriptional gene silencing for a number of model systems. Here, we describe selection of the target sequence for efficient RNAi knockdown in mouse.

More Related Videos

Rearing and Double-stranded RNA-mediated Gene Knockdown in the Hide Beetle, Dermestes maculatus
09:57

Rearing and Double-stranded RNA-mediated Gene Knockdown in the Hide Beetle, Dermestes maculatus

Published on: December 28, 2016

RNAi Interference by dsRNA Injection into Drosophila Embryos
08:30

RNAi Interference by dsRNA Injection into Drosophila Embryos

Published on: April 11, 2011

Related Experiment Videos

Last Updated: Jun 16, 2026

In ovo Electroporation of miRNA-based Plasmids in the Developing Neural Tube and Assessment of Phenotypes by DiI Injection in Open-book Preparations
12:41

In ovo Electroporation of miRNA-based Plasmids in the Developing Neural Tube and Assessment of Phenotypes by DiI Injection in Open-book Preparations

Published on: October 16, 2012

Rearing and Double-stranded RNA-mediated Gene Knockdown in the Hide Beetle, Dermestes maculatus
09:57

Rearing and Double-stranded RNA-mediated Gene Knockdown in the Hide Beetle, Dermestes maculatus

Published on: December 28, 2016

RNAi Interference by dsRNA Injection into Drosophila Embryos
08:30

RNAi Interference by dsRNA Injection into Drosophila Embryos

Published on: April 11, 2011

Related Concept Videos

Experimental RNAi02:15

Experimental RNAi

RNA interference (RNAi) is a cellular mechanism that inhibits gene expression by suppressing its transcription or activating the RNA degradation process. The mechanism was discovered by Andrew Fire and Craig Mello in 1998 in plants. Today, it is observed in almost all eukaryotes, including protozoa, flies, nematodes, insects, parasites, and mammals. This precise cellular mechanism of gene silencing has been developed into a technique that provides an efficient way to identify and determine the...
RNA Interference01:23

RNA Interference

RNA interference (RNAi) is a process in which a small non-coding RNA molecule blocks the post-transcriptional expression of a gene by binding to its messenger RNA (mRNA) and preventing the protein from being translated.
This process occurs naturally in cells, often through the activity of genomically-encoded microRNAs. Researchers can take advantage of this mechanism by introducing synthetic RNAs to deactivate specific genes for research or therapeutic purposes. For example, RNAi could be used...

Articles linked to this work by shared authors, journal, and citation graph.

Small RNA pathways in mammalian oocytes.

FEBS open bio·2026

Advances in the Use of Ionic Liquids as Smart Lubricants and Additives for Tribological Applications.

Materials (Basel, Switzerland)·2026

Phytocannabinoids as anti-inflammatory agents: Synergistic effects when combined with Cannabis sativa L. matrices.

Journal of ethnopharmacology·2026

Effect of the Incorporation of EuCl3 as a Fluorescent Probe into Ionic Liquids on Rheological and Electrical Properties for Tribological Applications.

ACS omega·2025

Artificial intelligence-assisted chest radiograph interpretation in Role 2 military field hospital settings: a controlled experimental study.

Trauma surgery & acute care open·2025

Response to "Antiviral RNA silencing in mammals: the importance of selecting the appropriate experimental model".

Nucleic acids research·2025

Procedures for Standardized Measurements of Core Traits in Maize Research and Breeding Programs.

Cold Spring Harbor protocols·2026

Standardized Trait Measurement in Maize Breeding and Genetics Research.

Cold Spring Harbor protocols·2026

Emerging Trends in Mass Spectrometry-Based Quantitative Proteome and Phosphoproteome Profiling in Maize.

Cold Spring Harbor protocols·2026

Sample Preparation for Quantitative Proteome and Phosphoproteome Profiling of Maize Tissues.

Cold Spring Harbor protocols·2026

High-Throughput Microbial Assay for Amino Acid Measurement in Ground Maize Seed Samples Utilizing Auxotrophic E. coli.

Cold Spring Harbor protocols·2025

Grain Quality in Maize.

Cold Spring Harbor protocols·2025

Regulating the Dynamic Properties of Knoevenagel Condensation Cross-Linked Hydrogels via Thia-Michael Addition Reaction.

Macromolecular rapid communications·2026

A Surface Engineering Strategy to Suppress Cathodic Precipitation in Seawater Electrolysis.

Small science·2026

Studies on plasmon-enhanced photocatalytic efficacy of thin Cu x O films for the degradation of imidacloprid.

Nanoscale advances·2026

2D Germanane-Based Z-Scheme Heterojunctions for Ultrafast Antibiotic Degradation and Mineralization.

Small science·2026

Leaching of Di(2-ethylhexyl) Phthalate from Polyvinyl Chloride Microplastics in Synthetic Freshwater, Seawater, and Fish In Vitro Digestion Fluids.

ACS ES&T water·2026

Adsorbed Oxygen-Induced d-p Orbital Hybridization for Piezocatalytic H2O2 Production, Antibiotic Degradation and Detoxification.

Small (Weinheim an der Bergstrasse, Germany)·2026
See all related articles
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies
Jove
Visualize
Contact Us