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Related Experiment Video

Updated: Jun 16, 2026

Labeling of Single Cells in the Central Nervous System of Drosophila melanogaster
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DiI cell labeling in lamprey embryos.

Natalya Nikitina1, Marianne Bronner-Fraser, Tatjana Sauka-Spengler

  • 1Division of Biology, California Institute of Technology, Pasadena, CA 91125, USA.

Cold Spring Harbor Protocols
|February 12, 2010
PubMed
Summary
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Lampreys, basal vertebrates, offer insights into vertebrate evolution. Microinjecting fluorescent dye into lamprey embryos enables detailed study of cell fate during development.

Area of Science:

  • Evolutionary Biology
  • Developmental Biology
  • Genomics

Background:

  • Lampreys possess key vertebrate features, making them crucial for understanding vertebrate origins.
  • Studying lampreys provides insights into the evolution of neural crest, placodes, and other vertebrate traits.
  • Lamprey embryos are ideal models for developmental studies due to their accessibility and amenability to techniques like microinjection.

Purpose of the Study:

  • To detail a microinjection protocol for labeling lamprey embryo cells.
  • To facilitate the study of cell fate and developmental mechanisms in early vertebrates.
  • To leverage lamprey's unique biology for insights into vertebrate evolution and parallel evolution.

Main Methods:

  • Microinjection of the fluorescent dye DiI (1,1'-dioctadecyl-3,3,3',3'-tetramethylindocarbocyanine perchlorate) into lamprey embryos.

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Related Experiment Videos

Last Updated: Jun 16, 2026

Labeling of Single Cells in the Central Nervous System of Drosophila melanogaster
10:33

Labeling of Single Cells in the Central Nervous System of Drosophila melanogaster

Published on: March 4, 2013

Cell Labeling and Injection in Developing Embryonic Mouse Hearts
07:20

Cell Labeling and Injection in Developing Embryonic Mouse Hearts

Published on: April 17, 2014

Labeling and Imaging Cells in the Zebrafish Hindbrain
09:17

Labeling and Imaging Cells in the Zebrafish Hindbrain

Published on: July 25, 2010

  • Utilizing the double chorion of lamprey embryos for efficient, dry-dish injections.
  • Leveraging large egg yields from individual lamprey females for high-throughput embryo manipulation.

    • Main Results:

    • The protocol enables precise labeling of individual cells within lamprey embryos.
    • Successful application of microinjection allows for detailed tracking of cell lineages during development.
    • Demonstrates the feasibility of studying cell fate in a basal vertebrate model.

    Conclusions:

    • This microinjection technique is a powerful tool for lamprey developmental studies.
    • Lamprey embryos serve as an excellent model for investigating the evolution of vertebrate development.
    • The method advances our understanding of cell fate mechanisms and evolutionary developmental biology.