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Updated: Jun 16, 2026
12:15
In Situ Hybridization for the Precise Localization of Transcripts in Plants
Published on: November 23, 2011
In situ hybridization in the plant Kalanchoë daigremontiana
1Department of Plant Biology, University of California, Davis, CA 95616, USA.
Cold Spring Harbor Protocols
|February 12, 2010
Abstract:
Here we describe in detail the detection of gene expression in plant tissues of Kalanchoë daigremontiana by in situ hybridization analyses. Included are methods for making RNA transcript probes, probe-tissue hybridization, and detection of antisense RNA probes. The in situ hybridization technique is used to determine which cells or group of cells in particular tissue(s) express a gene of interest.
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In situ hybridization (ISH) is a technique used to detect and localize specific DNA or RNA molecules in cells, tissue, or tissue sections using a labeled probe. The technique was first used in 1969 for the investigation of nucleic acids. It is currently an essential tool in scientific research and clinical settings, especially for diagnostic purposes.
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A probe is a complementary strand of DNA or RNA that binds to corresponding nucleotide sequences in a cell. Many...
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