Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Video

Updated: Jun 16, 2026

Retrograde Loading of Nerves, Tracts, and Spinal Roots with Fluorescent Dyes
06:47

Retrograde Loading of Nerves, Tracts, and Spinal Roots with Fluorescent Dyes

Published on: April 19, 2012

Dye loading with patch pipettes.

Jens Eilers, Arthur Konnerth

    Cold Spring Harbor Protocols
    |February 12, 2010
    PubMed
    Summary
    This summary is machine-generated.

    This protocol details loading single cells with fluorescent probes using patch pipettes. This versatile method enables combined electrophysiological and optical measurements for various cell studies.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Thalamic activation of the visual cortex at the single-synapse level.

    Science (New York, N.Y.)·2026
    Same author

    Cellular multipoint adaptive technology for two-photon mesoscope.

    Neurophotonics·2026
    Same author

    Glutamate indicators with increased sensitivity and tailored deactivation rates.

    Nature methods·2025
    Same author

    Ultrastructural membrane dynamics of mouse and human cortical synapses.

    Neuron·2025
    Same author

    Presynaptic ATP Decreases During Physiological-Like Activity in Neurons Tuned for High-Frequency Transmission.

    Journal of neurochemistry·2025
    Same author

    Amyloid β-dependent neuronal silencing through synaptic decoupling.

    Proceedings of the National Academy of Sciences of the United States of America·2025
    Same journal

    High-Throughput Microbial Assay for Amino Acid Measurement in Ground Maize Seed Samples Utilizing Auxotrophic <i>E. coli</i>.

    Cold Spring Harbor protocols·2025
    Same journal

    Grain Quality in Maize.

    Cold Spring Harbor protocols·2025
    Same journal

    High-Throughput Assay for Measuring Phytate and Available Phosphorus in Ground Maize Seed Samples.

    Cold Spring Harbor protocols·2025
    Same journal

    Functional Genomic Analysis of Transposon Insertion Mutant Maize Plants from the UniformMu National Public Resource.

    Cold Spring Harbor protocols·2025
    Same journal

    The UniformMu National Public Resource: Transposon<i>-</i>Induced Mutant Seeds for Functional Genomics Studies in Maize.

    Cold Spring Harbor protocols·2025
    Same journal

    Insights from the Study of B-Cell Epitopes of a Microbial Pathogen by Phage Display.

    Cold Spring Harbor protocols·2025
    See all related articles

    Area of Science:

    • Neuroscience
    • Cell Biology
    • Biophysics

    Background:

    • Patch-clamp technique is established for single-cell electrophysiology.
    • Fluorescent probes are crucial for visualizing cellular processes.
    • Combining electrophysiology with optical measurements offers deeper insights.

    Purpose of the Study:

    • To describe a protocol for loading individual cells with fluorescent probes using patch pipettes.
    • To highlight the versatility of this technique for various cell types and applications.
    • To emphasize the combined electrophysiological and optical measurement capabilities.

    Main Methods:

    • Utilizing whole-cell patch-clamp recordings for dye loading.
    • Employing patch pipettes for precise delivery of fluorescent probes into single cells.

    More Related Videos

    Imaging Exocytosis in Retinal Bipolar Cells with TIRF Microscopy
    11:54

    Imaging Exocytosis in Retinal Bipolar Cells with TIRF Microscopy

    Published on: June 9, 2009

    Single Cell Multiplex Reverse Transcription Polymerase Chain Reaction After Patch-clamp
    10:44

    Single Cell Multiplex Reverse Transcription Polymerase Chain Reaction After Patch-clamp

    Published on: June 20, 2018

    Related Experiment Videos

    Last Updated: Jun 16, 2026

    Retrograde Loading of Nerves, Tracts, and Spinal Roots with Fluorescent Dyes
    06:47

    Retrograde Loading of Nerves, Tracts, and Spinal Roots with Fluorescent Dyes

    Published on: April 19, 2012

    Imaging Exocytosis in Retinal Bipolar Cells with TIRF Microscopy
    11:54

    Imaging Exocytosis in Retinal Bipolar Cells with TIRF Microscopy

    Published on: June 9, 2009

    Single Cell Multiplex Reverse Transcription Polymerase Chain Reaction After Patch-clamp
    10:44

    Single Cell Multiplex Reverse Transcription Polymerase Chain Reaction After Patch-clamp

    Published on: June 20, 2018

  • Applying a broad range of fluorescent dyes suitable for patch-clamp loading.

    • Main Results:

    • Successful single-cell dye labeling in cultured neurons, brain slices, and in vivo preparations.
    • Demonstrated suitability of various dyes, including morphological markers, ion indicators, and FRET/FCS/FRAP probes.
    • Established Ca(2+) imaging as a primary application.

    Conclusions:

    • Whole-cell patch-clamp recording is a versatile technique for single-cell fluorescent probe loading.
    • This method facilitates combined electrophysiological and quantitative optical measurements.
    • The protocol supports diverse applications in cell biology and neuroscience research.