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Whole-mount in situ hybridization of honeybee (Apis mellifera) tissues

Peter K Dearden1, Elizabeth J Duncan, Megan J Wilson

  • 1Laboratory for Evolution and Development, National Research Centre for Growth and Development, Biochemistry Department, University of Otago, Dunedin 9054, New Zealand. peter.dearden@otago.ac.nz

Cold Spring Harbor Protocols
|February 12, 2010
PubMed
Abstract

No abstract available in PubMed .

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In-situ Hybridization02:31

In-situ Hybridization

In situ hybridization (ISH) is a technique used to detect and localize specific DNA or RNA molecules in cells, tissue, or tissue sections using a labeled probe. The technique was first used in 1969 for the investigation of nucleic acids. It is currently an essential tool in scientific research and clinical settings, especially for diagnostic purposes.
Types of probes and labels
A probe is a complementary strand of DNA or RNA that binds to corresponding nucleotide sequences in a cell. Many...