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Related Concept Videos

Mass Spectrometry: Isotope Effect01:13

Mass Spectrometry: Isotope Effect

Most elements exist in nature as a mixture of isotopes. The isotopes differ in weight due to their respective number of neutrons. The molecular weight of a molecule is different depending on the specific isotope of its elements involved. As a result, the mass spectrum of the molecule exhibits peaks from the same fragment at multiple positions. The positions of these mass signals depend on the mass differences between isotopes. Furthermore, the intensity of these signals is dependent on the...
Isotopes01:12

Isotopes

Elements have a set number of protons that determines their atomic number (Z). For example, all atoms with eight protons are oxygen; however, the number of neutrons can vary for atoms of the same element. The sum of the number of protons and the number of neutrons is the mass number (A). Atoms with the same atomic number but different mass numbers are called isotopes. Elements can have multiple isotopes, for example, carbon-12, carbon-13, and carbon-14.An element's atomic mass, or weight, is a...
Atomic Emission Spectroscopy: Overview01:20

Atomic Emission Spectroscopy: Overview

Atomic emission spectroscopy (AES) is an analytical technique used to determine the elemental composition of a sample by analyzing the light emitted from excited atoms. In AES, atoms in a sample are excited to higher energy levels by thermal energy from high-temperature sources, such as plasma, arcs, or sparks. When these excited atoms return to lower energy states, they emit light at specific wavelengths characteristic of each element. The resulting atomic emission spectrum, which consists of...
Atomic Absorption Spectroscopy: Lab01:21

Atomic Absorption Spectroscopy: Lab

For AAS measurements, samples must be introduced as clear solutions, often requiring extensive preliminary treatment to dissolve materials like soils, animal tissues, and minerals. Common methods for sample preparation include treatment with hot mineral acids, wet ashing, combustion in closed containers, high-temperature ashing, or fusion with reagents.
 Solutions containing organic solvents, such as low-molecular-mass alcohols, esters, or ketones, enhance absorbances by increasing nebulizer...
Atomic Emission Spectroscopy: Lab01:29

Atomic Emission Spectroscopy: Lab

AES is a powerful analytical technique, especially effective when used with plasma sources, producing abundant spectra in characteristic emission lines. The Inductively Coupled Plasma (ICP), in particular, yields superior quantitative analytical data due to its high stability, low noise, low background, and minimal interferences under optimal experimental conditions. However, newer air-operated microwave sources are emerging as promising alternatives that could be more cost-effective than...
Elements: Chemical Symbols and Isotopes02:31

Elements: Chemical Symbols and Isotopes

A chemical symbol is an abbreviation used to indicate an element or an atom of an element. For example, the symbol for mercury is Hg. The same symbol is used to indicate one atom of mercury (microscopic domain) or to label a container of many atoms of the element mercury (macroscopic domain).
Some symbols are derived from the common English name of the element; others are abbreviations of the name in another language — Latin, Greek or German. For example, the symbol for aluminum (common name)...

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Related Experiment Video

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Stable Isotopic Profiling of Intermediary Metabolic Flux in Developing and Adult Stage Caenorhabditis elegans
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Published on: February 27, 2011

Stable isotope and elemental analysis in ants.

Chris R Smith1, Chadwick V Tillberg

  • 1School of Life Sciences, Arizona State University, Tempe, AZ 85287, USA. csmith38@asu.edu

Cold Spring Harbor Protocols
|February 12, 2010
PubMed
Summary
This summary is machine-generated.

This study presents a protocol for preparing ant samples for stable isotope analysis, aiding in understanding their feeding ecology and role in ecosystems. This method supports high-throughput analysis for ecological stoichiometry research.

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Area of Science:

  • Ecology
  • Biogeochemistry

Background:

  • Stable isotope analysis is increasingly used to study feeding ecology and ecological stoichiometry.
  • Ants are ecologically diverse and dominant, making them suitable models for dietary studies.
  • High-throughput stable isotope analysis requires specific sample preparation protocols.

Purpose of the Study:

  • To describe a protocol for preparing ant samples for natural abundance stable isotope analysis.
  • To facilitate high-throughput analysis of ecological stoichiometry in ants.

Main Methods:

  • The protocol focuses on sample preparation for stable isotope quantification.
  • It is designed to produce samples suitable for specialized high-throughput laboratories.
  • Specific lab requirements should be confirmed prior to sample submission.

Main Results:

  • The described protocol yields samples adequate for stable isotope analysis.
  • This method is applicable to natural abundance studies.
  • It does not cover trace sample preparation.

Conclusions:

  • This protocol provides a standardized method for ant sample preparation for stable isotope analysis.
  • It will aid researchers in exploring ant feeding ecology and their role in food webs.
  • The method supports advancements in ecological stoichiometry research.