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Related Concept Videos

In-situ Hybridization02:31

In-situ Hybridization

In situ hybridization (ISH) is a technique used to detect and localize specific DNA or RNA molecules in cells, tissue, or tissue sections using a labeled probe. The technique was first used in 1969 for the investigation of nucleic acids. It is currently an essential tool in scientific research and clinical settings, especially for diagnostic purposes.
Types of probes and labels
A probe is a complementary strand of DNA or RNA that binds to corresponding nucleotide sequences in a cell. Many...
FISH - Fluorescent In-situ Hybridization02:07

FISH - Fluorescent In-situ Hybridization

Fluorescence in situ hybridization, or FISH, was developed in the early 1980s and has quickly become one of the most widely used techniques in cytogenetics. Labeled probes are used to bind complementary DNA or RNA sequences on a chromosome or in a region within a cell. Earlier, the probes could only be obtained by cloning or reverse transcription of a DNA template. Currently, the probe oligonucleotides can be synthesized synthetically. Additionally, with the advancement of optical techniques,...

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Related Experiment Video

Updated: Jun 16, 2026

Wholemount In Situ Hybridization for Astyanax Embryos
09:56

Wholemount In Situ Hybridization for Astyanax Embryos

Published on: March 2, 2019

Amphioxus whole-mount in situ hybridization

J K Sky Yu1, Linda Z Holland

  • 1Institute of Cellular and Organismic Biology, Academia Sinica, Nankang, Taipei 11529, Taiwan, Republic of China. jkyu@gate.sinica.edu.tw

Cold Spring Harbor Protocols
|February 12, 2010
PubMed
Summary

No abstract available in PubMed .

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