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Immunohistochemical distribution of fibronectin in the mucosa with chronic sinusitis
Abstract:
The distribution of fibronectin (FN) in operative specimens of maxillary sinus mucosa from 35 patients with chronic sinusitis was examined immunohistochemically. Mouse monoclonal antibody to human FN was used for the peroxidase-antiperoxidase staining technique. FN was found in most of the serous cells and in some (about 10%) of the mucous cells in the nasal glands, and also in the nasal fluid in the lumina. It was not found elsewhere, be it in the epithelial cells, the basement membrane, or the interstitial tissue, except in two patients. These results suggest that FN is produced in the nasal glands and secreted into the nasal fluid in the maxillary sinus in chronic sinusitis.
Insights
Fibronectin (FN) is primarily found in nasal glands and fluid within the maxillary sinus during chronic sinusitis. This suggests nasal glands produce and secrete FN in this condition.
Area of Science:
- Immunohistochemistry
- Cell Biology
- Otorhinolaryngology
Background:
- Chronic sinusitis is a common condition affecting the maxillary sinus.
- The role of fibronectin (FN) in the pathophysiology of chronic sinusitis is not fully understood.
Purpose of the Study:
- To investigate the distribution of fibronectin (FN) in the maxillary sinus mucosa of patients with chronic sinusitis.
- To determine the cellular origin and location of FN within the affected sinus tissue.
Main Methods:
- Immunohistochemical analysis using a mouse monoclonal antibody to human FN.
- Examination of operative specimens from 35 patients diagnosed with chronic sinusitis.
- Peroxidase-antiperoxidase staining technique was employed.
Main Results:
- Fibronectin (FN) was predominantly detected in serous cells and, to a lesser extent, in mucous cells of the nasal glands.
- FN was also identified within the nasal fluid in the lumina of the maxillary sinus.
- FN was notably absent in epithelial cells, basement membrane, and interstitial tissue, with exceptions in two patients.
Conclusions:
- The findings indicate that nasal glands are a significant source of fibronectin (FN) production in the maxillary sinus.
- Fibronectin (FN) is secreted into the nasal fluid, suggesting a potential role in the inflammatory processes of chronic sinusitis.