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Site Directed Spin Labeling and EPR Spectroscopic Studies of Pentameric Ligand-Gated Ion Channels
Published on: July 4, 2016
Frequency Dependence of Pulsed EPR Experiments
Sandra S Eaton1, Gareth R Eaton
1Department of Chemistry and Biochemistry, University of Denver, Denver, CO, USA 80208.
Abstract:
The frequency dependence of the signal-to-noise ratio (S/N) that is theoretically possible for pulsed EPR experiments is the same as for continuous wave experiments. To select the optimum resonance frequency or frequencies for pulsed EPR experiments it is important to consider not only S/N, but also orientation selection, depth of spin echo modulation, and intensities of forbidden transitions. Evaluation of factors involved in selecting the optimum frequency for pulsed EPR measurements of distances between spins is discussed.
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