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Application of Mouse Parthenogenetic Haploid Embryonic Stem Cells as a Substitute of Sperm

Application of Mouse Parthenogenetic Haploid Embryonic Stem Cells as a Substitute of Sperm

Published on: November 19, 2020

Generation of genetically modified animals using spermatogonial stem cells.

Masanori Takehashi¹, Mito Kanatsu-Shinohara, Takashi Shinohara

¹Faculty of Pharmacy, Osaka Ohtani University, Tondabayashi, Osaka, Japan. takeham@osaka-ohtani.ac.jp

Development, Growth & Differentiation

|February 13, 2010

Summary

Spermatogonial stem cells (SSCs) can be cultured as germline stem (GS) cells and multipotent GS (mGS) cells. These cells enable efficient gene targeting in mice and offer potential for regenerative medicine, avoiding ethical and immunological issues.

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Serial Enrichment of Spermatogonial Stem and Progenitor Cells (SSCs) in Culture for Derivation of Long-term Adult Mouse SSC Lines

Serial Enrichment of Spermatogonial Stem and Progenitor Cells (SSCs) in Culture for Derivation of Long-term Adult Mouse SSC Lines

Published on: February 25, 2013

Area of Science:

Stem Cell Biology
Reproductive Biology
Gene Targeting Technology

Background:

Spermatogonial stem cells (SSCs) are crucial for spermatogenesis and genetic transmission to offspring.
Established protocols allow for the transplantation and long-term culture of SSCs as germline stem (GS) cells.
SSCs possess unique potential, exhibiting both spermatogenic capacity and pluripotency akin to embryonic stem (ES) cells.

Purpose of the Study:

To explore the potential of GS and multipotent GS (mGS) cells in animal transgenesis.
To evaluate mGS cells as an alternative to ES cells for generating genetically modified animals.
To assess the utility of mGS cells in regenerative medicine, addressing limitations of ES and induced pluripotent stem cells.

Main Methods:

Culture and transplantation of mouse SSCs to establish GS and mGS cell lines.
Generation of knockout mice using GS and mGS cells.
Comparison of mGS cell capabilities with ES cells and other gene-targeting methods.

Main Results:

GS and mGS cells were successfully established and utilized for generating knockout mice.
mGS cells demonstrated pluripotency comparable to ES cells, enabling efficient transgenesis.
The study highlights the potential of mGS cells for applications in regenerative medicine.

Conclusions:

GS and mGS cells represent a significant advancement in gene-targeting technology for animal models.
mGS cells offer a promising alternative to ES cells, circumventing ethical and immunological concerns.
The application of mGS cells in regenerative medicine holds potential for overcoming challenges associated with current stem cell therapies.