Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Extrinsic and Intrinsic Pathways of Hemostasis01:20

Extrinsic and Intrinsic Pathways of Hemostasis

Blood clotting or coagulation involves extrinsic and intrinsic pathways, which ultimately merge into the common pathway, forming a fibrin clot.
The Extrinsic Pathway
The extrinsic pathway of coagulation is typically initiated by tissue damage that exposes blood to tissue factor (TF), a protein released by the damaged tissue cells outside the blood vessels—this interaction with TF triggers biochemical reactions involving specific clotting factors. The key player here is Factor VII, which forms a...
Transcription Factors02:16

Transcription Factors

Tissue-specific transcription factors contribute to diverse cellular functions in mammals. For example, the gene for beta globin, a major component of hemoglobin, is present in all cells of the body. However, it is only expressed in red blood cells because the transcription factors that can bind to the promoter sequences of the beta globin gene are only expressed in these cells. Tissue-specific transcription factors also ensure that mutations in these factors may impair only the function of...
General Transcription Factors01:30

General Transcription Factors

Tissue-specific transcription factors contribute to diverse cellular functions in mammals. For example, the gene for beta globin, a major component of hemoglobin, is present in all cells of the body. However, it is only expressed in red blood cells because the transcription factors that can bind to the promoter sequences of the beta globin gene are only expressed in these cells. Tissue-specific transcription factors also ensure that mutations in these factors may impair only the function of...
Intracellular Signaling Affects Focal Adhesions01:17

Intracellular Signaling Affects Focal Adhesions

Integrins act both as extracellular input receivers and as intracellular processing activators. As their name suggests, integrins are entirely integrated into the membrane structure. Their hydrophobic membrane-spanning regions interact with the phospholipid bilayer's hydrophobic region. These membrane receptors provide extracellular attachment sites for effectors like hormones and growth factors. They activate intracellular response cascades when their effectors are bound and active.
Some...
Regulation of Angiogenesis and Blood Supply01:24

Regulation of Angiogenesis and Blood Supply

Rapidly dividing tumors, embryos, and wounded tissues require more oxygen than usual, lowering the oxygen concentration in the blood. At low oxygen or hypoxic conditions, an oxygen-sensitive transcription factor called the hypoxia-inducible factor 1 or HIF1 is activated. HIF1 is a dimeric protein of alpha (ɑ) and beta (β) subunits.  Under optimal oxygen conditions, HIF1β is present in the nucleus while HIF1ɑ remains in the cytosol. HIF1ɑ is hydroxylated by prolyl hydroxylase and factor...
Coagulation01:09

Coagulation

The coagulation phase is a critical part of the body's process to prevent blood loss following injury to blood vessels. It involves chemical reactions that form a clot to seal the injured area. The clotting process begins shortly after injury, within 15-20 seconds for severe damage and 1-2 minutes for minor injuries.
During the coagulation phase, clotting factors, or procoagulants, play a vital role in initiating and progressing the coagulation cascade. This cascade is a series of reactions...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Revisiting Platelet-Associated Tissue Factor: Methodological Concerns in Flow Cytometry Detection.

Thrombosis and haemostasis·2026
Same author

Oral contraceptives and tissue factor expression. Comments on "Do combined oral contraceptives induce formation of tissue factor?"

Research and practice in thrombosis and haemostasis·2026
Same author

Comparison of assays measuring extracellular vesicle tissue factor in plasma samples: communication from the ISTH SSC Subcommittee on Vascular Biology.

Journal of thrombosis and haemostasis : JTH·2024
Same author

Extracellular vesicles from activated platelets possess a phospholipid-rich biomolecular profile and enhance prothrombinase activity.

Journal of thrombosis and haemostasis : JTH·2024
Same author

"A rapid, sensitive, and specific assay to measure tissue factor activity based on chromogenic determination of thrombin generation": reply.

Journal of thrombosis and haemostasis : JTH·2023
Same author

Enhanced Blood Clotting After Rewarming From Experimental Hypothermia in an Intact Porcine Model.

Frontiers in physiology·2022

Related Experiment Video

Updated: Jun 16, 2026

Flow Cytometry Analysis of Tissue Factor Expression in Human Platelets
10:08

Flow Cytometry Analysis of Tissue Factor Expression in Human Platelets

Published on: November 22, 2024

Tissue factor expression in blood cells.

Bjarne Østerud1

  • 1Department of Medical Biology, Faculty of Health Sciences, University of Tromsø, Tromsø, Norway. bjarne.osterud@uit.no

Thrombosis Research
|February 13, 2010
PubMed
Summary
This summary is machine-generated.

Blood-borne tissue factor (TF) can induce thrombus formation. Monocytes are the primary source of TF in blood, with platelets potentially activating TF under specific conditions, challenging previous endothelial-centric views.

More Related Videos

Extracellular Vesicle Tissue Factor Activity Assay
03:53

Extracellular Vesicle Tissue Factor Activity Assay

Published on: December 29, 2023

A Microfluidic Flow Chamber Model for Platelet Transfusion and Hemostasis Measures Platelet Deposition and Fibrin Formation in Real-time
09:38

A Microfluidic Flow Chamber Model for Platelet Transfusion and Hemostasis Measures Platelet Deposition and Fibrin Formation in Real-time

Published on: February 14, 2017

Related Experiment Videos

Last Updated: Jun 16, 2026

Flow Cytometry Analysis of Tissue Factor Expression in Human Platelets
10:08

Flow Cytometry Analysis of Tissue Factor Expression in Human Platelets

Published on: November 22, 2024

Extracellular Vesicle Tissue Factor Activity Assay
03:53

Extracellular Vesicle Tissue Factor Activity Assay

Published on: December 29, 2023

A Microfluidic Flow Chamber Model for Platelet Transfusion and Hemostasis Measures Platelet Deposition and Fibrin Formation in Real-time
09:38

A Microfluidic Flow Chamber Model for Platelet Transfusion and Hemostasis Measures Platelet Deposition and Fibrin Formation in Real-time

Published on: February 14, 2017

Area of Science:

  • Hematology
  • Vascular Biology
  • Immunology

Background:

  • The traditional view positions tissue factor (TF) primarily as a component of the vascular subendothelium involved in hemostasis.
  • Recent findings suggest blood-borne TF contributes to thrombus formation, particularly under conditions of shear stress and activated platelets.

Purpose of the Study:

  • To investigate the cellular sources and regulation of tissue factor (TF) activity in blood.
  • To clarify the role of monocytes, platelets, and endothelial cells in TF-mediated thrombosis.

Main Methods:

  • Utilized sensitive assays to measure TF activity in various blood cells (monocytes, neutrophils, eosinophils, platelets) and microparticles.
  • Investigated TF expression and activity following stimulation with agonists and under conditions of shear stress.
  • Examined the interaction between activated platelets and monocytes in TF decryption.

Main Results:

  • Monocytes were identified as the sole blood cells synthesizing and expressing detectable TF activity.
  • No significant TF activity was found in activated platelets, though they may facilitate monocyte TF activation.
  • Evidence for in vivo TF synthesis by endothelial cells in thrombosis models was limited.

Conclusions:

  • Monocytes are the primary and potentially exclusive source of blood-borne TF responsible for TF-induced thrombosis when the endothelium is intact.
  • Activated platelets may play a role in modulating monocyte-derived TF activity rather than being a direct source of TF.