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Related Concept Videos

Protein Folding01:25

Protein Folding

Proteins are chains of amino acids linked together by peptide bonds. Upon synthesis, a protein folds into a three-dimensional conformation, critical to its biological function. Interactions between its constituent amino acids guide protein folding, and hence the protein structure is primarily dependent on its amino acid sequence.
Protein Structure Is Critical to Its Biological Function
Proteins perform a wide range of biological functions such as catalyzing chemical reactions, providing...
Protein Folding01:22

Protein Folding

Overview
Protein Folding01:22

Protein Folding

Overview
Molecular Chaperones and Protein Folding03:00

Molecular Chaperones and Protein Folding

The native conformation of a protein is formed by interactions between the side chains of its constituent amino acids. When the amino acids cannot form these interactions, the protein cannot fold by itself and needs chaperones. Notably, chaperones do not relay any additional information required for the folding of polypeptides; the native conformation of a protein is determined solely by its amino acid sequence. Chaperones catalyze protein folding without being a part of the folded protein.
The...
Molecular Chaperones and Protein Folding03:00

Molecular Chaperones and Protein Folding

The native conformation of a protein is formed by interactions between the side chains of its constituent amino acids. When the amino acids cannot form these interactions, the protein cannot fold by itself and needs chaperones. Notably, chaperones do not relay any additional information required for the folding of polypeptides; the native conformation of a protein is determined solely by its amino acid sequence. Chaperones catalyze protein folding without being a part of the folded protein.
The...
Proteins: From Genes to Degradation02:11

Proteins: From Genes to Degradation

Within a biological system, the DNA encodes the RNA, and the nucleotide sequence in the RNA further defines the amino acid sequence in the protein. This is referred to as “The Central Dogma of Molecular Biology” - a term coined by Francis Crick.  Central dogma is a firm principle in biology that defines the flow of genetic information within any life form. The two fundamental steps in central dogma are - transcription and translation.
Transcription is the synthesis of RNA molecules by RNA...

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Related Experiment Video

Updated: Jun 16, 2026

Using SecM Arrest Sequence as a Tool to Isolate Ribosome Bound Polypeptides
09:42

Using SecM Arrest Sequence as a Tool to Isolate Ribosome Bound Polypeptides

Published on: June 19, 2012

Protein folding on the ribosome.

Lisa D Cabrita1, Christopher M Dobson, John Christodoulou

  • 1Department of Chemistry, University of Cambridge, Cambridge, United Kingdom.

Current Opinion in Structural Biology
|February 13, 2010
PubMed
Summary

Newly synthesized proteins must fold correctly to function. This review explores recent methods and current understanding of how polypeptide chains fold after emerging from ribosomes.

Area of Science:

  • Molecular biology
  • Biochemistry
  • Structural biology

Background:

  • Polypeptide chains are synthesized on ribosomes, complex molecular machines.
  • Nascent polypeptide chains emerging from ribosomes require proper folding to become functional.
  • Protein folding is a critical process for cellular function.

Purpose of the Study:

  • To discuss recent approaches for studying protein folding.
  • To review current knowledge on how polypeptide chains fold.
  • To elucidate the complex self-assembly process of protein folding.

Main Methods:

  • Review of recent experimental and computational approaches.
  • Analysis of techniques revealing in-cell protein folding dynamics.
  • Synthesis of findings from diverse research methodologies.

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Analysis of Protein Folding, Transport, and Degradation in Living Cells by Radioactive Pulse Chase
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Analysis of Protein Folding, Transport, and Degradation in Living Cells by Radioactive Pulse Chase

Published on: February 12, 2019

Single Molecule Fluorescence Energy Transfer Study of Ribosome Protein Synthesis
08:07

Single Molecule Fluorescence Energy Transfer Study of Ribosome Protein Synthesis

Published on: July 6, 2021

Related Experiment Videos

Last Updated: Jun 16, 2026

Using SecM Arrest Sequence as a Tool to Isolate Ribosome Bound Polypeptides
09:42

Using SecM Arrest Sequence as a Tool to Isolate Ribosome Bound Polypeptides

Published on: June 19, 2012

Analysis of Protein Folding, Transport, and Degradation in Living Cells by Radioactive Pulse Chase
08:59

Analysis of Protein Folding, Transport, and Degradation in Living Cells by Radioactive Pulse Chase

Published on: February 12, 2019

Single Molecule Fluorescence Energy Transfer Study of Ribosome Protein Synthesis
08:07

Single Molecule Fluorescence Energy Transfer Study of Ribosome Protein Synthesis

Published on: July 6, 2021

Main Results:

  • Emerging methods provide new insights into cotranslational and posttranslational folding.
  • Understanding of factors influencing folding pathways is advancing.
  • The complexity of protein self-assembly is being increasingly appreciated.

Conclusions:

  • Continued development of advanced techniques is crucial for understanding protein folding.
  • A comprehensive understanding of protein folding is essential for molecular and cellular biology.
  • This review consolidates current knowledge and highlights future research directions.