Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Extrinsic and Intrinsic Pathways of Hemostasis01:20

Extrinsic and Intrinsic Pathways of Hemostasis

Blood clotting or coagulation involves extrinsic and intrinsic pathways, which ultimately merge into the common pathway, forming a fibrin clot.
The Extrinsic Pathway
The extrinsic pathway of coagulation is typically initiated by tissue damage that exposes blood to tissue factor (TF), a protein released by the damaged tissue cells outside the blood vessels—this interaction with TF triggers biochemical reactions involving specific clotting factors. The key player here is Factor VII, which forms a...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

von Willebrand Factor fibers formed at pathological high shear provide a scaffold for α-synuclein binding and aggregation.

Scientific reports·2026
Same author

Understanding how a highly prevalent GRK5 polymorphism affects platelets and enhances thrombotic risk.

Blood·2026
Same author

From imaging to computational domains for physics-driven molecular biology simulations: Hindered diffusion in platelet masses.

PLoS computational biology·2025
Same author

Multiscale simulations that incorporate patient-specific neural network models of platelet calcium signaling predict diverse thrombotic outcomes under flow.

PLoS computational biology·2025
Same author

Rapid Determination of Xa Inhibitor Activity in Blood Using a Microfluidic Device that Measures Platelet Deposition and Fibrin Generation Under Flow.

TH open : companion journal to thrombosis and haemostasis·2025
Same author

From imaging to computational domains for physics-driven molecular biology simulations: Hindered diffusion in platelet masses.

bioRxiv : the preprint server for biology·2025
Same journal

Heatstroke-induced coagulopathy: A scoping review of therapeutic strategies and outcome reporting.

Thrombosis research·2026
Same journal

Mapping thrombus habitat: Non-contrast MRI radiomics and pixel-tile histomics approach to track venous thrombosis evolution in mice.

Thrombosis research·2026
Same journal

A study protocol for a randomised controlled trial evaluating the safety and efficiency of the YEARS algorithm versus computed tomography pulmonary angiography only for suspected acute pulmonary embolism in patients with cancer: the Hydra Study.

Thrombosis research·2026
Same journal

Associating the phenotypic expression of platelets with disease type through image-based single-cell profiling.

Thrombosis research·2026
Same journal

The mechanisms of contractile dysfunction following chronic limited platelet activation in (pro)thrombotic conditions.

Thrombosis research·2026
Same journal

Molecular basis of the E69Q and R383W heterozygous F2 variants identified in the proband associated with severe hemostatic defects.

Thrombosis research·2026
See all related articles

Related Experiment Video

Updated: Jun 16, 2026

A Microfluidic Flow Chamber Model for Platelet Transfusion and Hemostasis Measures Platelet Deposition and Fibrin Formation in Real-time
09:38

A Microfluidic Flow Chamber Model for Platelet Transfusion and Hemostasis Measures Platelet Deposition and Fibrin Formation in Real-time

Published on: February 14, 2017

Tissue factor activity under flow.

Scott L Diamond1

  • 1Institute for Medicine and Engineering, Department of Chemical and Biomolecular Engineering, University of Pennsylvania, Philadelphia, PA 19104, USA. sld@seas.upenn.edu

Thrombosis Research
|February 13, 2010
PubMed
Summary
This summary is machine-generated.

Blood flow significantly alters coagulation compared to static tests. High platelet concentrations form at clot sites in flowing blood, impacting drug efficacy and thrombosis.

More Related Videos

Flow Cytometry Analysis of Tissue Factor Expression in Human Platelets
10:08

Flow Cytometry Analysis of Tissue Factor Expression in Human Platelets

Published on: November 22, 2024

Extracellular Vesicle Tissue Factor Activity Assay
03:53

Extracellular Vesicle Tissue Factor Activity Assay

Published on: December 29, 2023

Related Experiment Videos

Last Updated: Jun 16, 2026

A Microfluidic Flow Chamber Model for Platelet Transfusion and Hemostasis Measures Platelet Deposition and Fibrin Formation in Real-time
09:38

A Microfluidic Flow Chamber Model for Platelet Transfusion and Hemostasis Measures Platelet Deposition and Fibrin Formation in Real-time

Published on: February 14, 2017

Flow Cytometry Analysis of Tissue Factor Expression in Human Platelets
10:08

Flow Cytometry Analysis of Tissue Factor Expression in Human Platelets

Published on: November 22, 2024

Extracellular Vesicle Tissue Factor Activity Assay
03:53

Extracellular Vesicle Tissue Factor Activity Assay

Published on: December 29, 2023

Area of Science:

  • Hemostasis and Thrombosis
  • Biomedical Engineering
  • Pharmacology

Background:

  • Intravascular thrombosis occurs in an open system with continuous blood flow, unlike static laboratory models.
  • Red blood cell migration in vessels concentrates platelets near the wall, significantly increasing local platelet counts.
  • Existing laboratory methods for evaluating coagulation and anti-platelet agents often fail to replicate flow dynamics.

Purpose of the Study:

  • To highlight the fundamental differences between coagulation under flow and static conditions.
  • To emphasize the impact of high, flow-induced platelet concentrations on thrombosis and drug efficacy.
  • To underscore the limitations of current laboratory models in simulating open, flowing systems.

Main Methods:

  • Utilizing controlled in vitro whole blood perfusion systems.
  • Investigating platelet accumulation and concentration dynamics at thrombotic surfaces under flow.
  • Assessing the critical threshold of surface tissue factor for triggering coagulation.
  • Evaluating the potency of anti-platelet agents relative to wall shear rate.

Main Results:

  • Platelet concentrations at a thrombus surface can reach 10-50 times higher than in platelet-rich plasma due to flow.
  • Convective transport in open systems influences the delivery and removal of species, affecting drug efficacy.
  • In vitro perfusion systems can determine the minimum tissue factor required for coagulation on collagen.
  • The potency of anti-platelet therapies can be characterized as a function of wall shear rate.

Conclusions:

  • Coagulation under flow conditions presents unique challenges and mechanisms distinct from static clotting.
  • Flow-driven platelet accumulation and convective transport are critical factors in intravascular thrombosis.
  • In vitro perfusion models offer a more physiologically relevant platform for studying thrombosis and evaluating antithrombotic agents.