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pBaSysBioII: an integrative plasmid generating gfp transcriptional fusions for high-throughput analysis of gene

Eric Botella1, Mark Fogg2, Matthieu Jules3

  • 1Smurfit Institute of Genetics, Trinity College Dublin, Dublin 2, Ireland.

Microbiology (Reading, England)
|February 13, 2010
PubMed

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Reporter Genes02:11

Reporter Genes

Reporter genes are a type of protein-coding gene that are often tagged to a gene of interest. Once inside a target cell, reporter genes usually produce visually identifiable characteristics like fluorescence and luminescence when expressed along with the gene of interest. Thus, reporter genes “report” the presence or absence of genes of interest in an organism, determine the gene expression pattern, or track the physical location of a DNA segment or protein in the cell.
Commonly used reporter...