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Transplantation of Induced Pluripotent Stem Cell-derived Mesoangioblast-like Myogenic Progenitors in Mouse Models of Muscle Regeneration
Published on: January 20, 2014
Reconstitution of experimental neurogenic bladder dysfunction using skeletal muscle-derived multipotent stem cells
Masahiro Nitta1, Tetsuro Tamaki, Kayoko Tono
1Department of Urology, Division of Surgery, Tokai University School of Medicine, Shimokasuya, Isehara, Kanagawa 259-1143, Japan.
Transplantation
|February 13, 2010
Summary
Skeletal muscle stem cells (Sk-34 and Sk-DN) can restore bladder function after surgery by repairing damaged nerves and blood vessels. This approach shows promise for treating neurogenic bladder dysfunction following cervical cancer treatment.
Area of Science:
- Regenerative Medicine
- Urology
- Oncology
Background:
- Postoperative neurogenic bladder dysfunction is a significant complication of radical hysterectomy for cervical cancer.
- This dysfunction results from damage to the bladder branch of the pelvic plexus (BBPP) and associated blood vessels.
Purpose of the Study:
- To evaluate the potential of skeletal muscle-derived multipotent stem cells (Sk-34 and Sk-DN) to reconstitute damaged BBPP and restore bladder function.
- To assess the capacity of these stem cells to regenerate vascular, muscular, and nervous systems.
Main Methods:
- An experimental neurogenic bladder model was created by unilaterally cutting the BBPP and blood vessels in rats.
- Skeletal muscle-derived stem cells (Sk-34 and Sk-DN) were autografted around the damaged area.
- Bladder function was measured by intravesical pressure via electrical stimulation before and after transplantation.
- Morphological contribution of transplanted cells was examined using immunohistochemistry and immunoelectron microscopy.
Main Results:
- Stem cell transplantation resulted in significant functional recovery (approximately 80%) at 4 weeks post-surgery.
- Control groups showed minimal recovery (approximately 28% and 24%).
- Transplanted cells differentiated into Schwann cells, perineurial cells, vascular smooth muscle cells, pericytes, and fibroblasts, integrating into damaged nerves and blood vessels.
Conclusions:
- Multipotent stem cells (Sk-34 and Sk-DN) derived from skeletal muscle are effective in reconstituting damaged BBPP.
- This stem cell therapy holds potential for treating neurogenic bladder dysfunction after radical hysterectomy.
