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Updated: Jun 16, 2026

Grafting of Beads into Developing Chicken Embryo Limbs to Identify Signal Transduction Pathways Affecting Gene Expression
Published on: January 17, 2016
Isl-1 down-regulates DRG cell proliferation during chicken embryo development
Dawei Chen1, Guoxin Wang, Haoshu Luo
1State Key Laboratory of Agrobiotechnology, College of Biological Sciences, China Agricultural University, Beijing, China.
Objective:
Protein Isl-1 RNA interference and over expression in early chicken embryo dorsal root ganglia (DRG) were used to investigate the function of Isl-1 in DRG cell proliferation.
Methods:
Isl-1 targeted shRNA expression vector and Isl-1 over-expression vector were transfected into chicken embryo DRG by in ovo electroporation. Then, the DRG proliferation rate was detected by BrdU immunohistochemistry.
Results:
The rate of DRG cell proliferation increased after Isl-1 knock-down and decreased after Isl-1 over-expression.
Conclusions:
In this study, we found that Isl-1 negatively modulates DRG cell proliferation.
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