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A Rapid Method for Multispectral Fluorescence Imaging of Frozen Tissue Sections
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NorthernLights in slide-based cytometry and microscopy.

J T Wessels1, A C Busse, J Mahrt

  • 1Department Nephrology/Rheumatology, University Medicine, Goettingen, Germany. johannes.wessels@med.uni-goettingen.de

Cytometry. Part a : the Journal of the International Society for Analytical Cytology
|February 13, 2010
PubMed
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New NorthernLights secondary antibodies offer bright, photostable, and affordable fluorochrome options for slide-based cytometry and microscopy. These dyes, similar to common fluorochromes, enhance multiparametric analysis in various fluorescence techniques.

Area of Science:

  • Biotechnology
  • Cellular Imaging
  • Immunofluorescence

Background:

  • Slide-based cytometry is crucial for polychromatic investigations, driving demand for more measurable fluorochromes.
  • Photostability is key for multiparametric analysis, with ALEXA dyes showing superior performance over conventional fluorochromes like FITC, PE, and APC.

Purpose of the Study:

  • To evaluate NorthernLights secondary antibodies for slide-based cytometry and microscopy.
  • To assess the spectral properties and photostability of NorthernLights fluorochromes (NL493, NL557, NL637).

Main Methods:

  • Analysis of NorthernLights secondary antibodies with distinct excitation and emission maxima.
  • Comparison of spectral properties with established fluorochromes (FITC, PE, APC).
  • Assessment of photobleaching resistance, stability in alcohols/xylene, and brightness.

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Main Results:

  • NorthernLights antibodies exhibit spectral properties comparable to FITC, PE, and APC.
  • These fluorochromes demonstrate high brightness and resistance to photobleaching.
  • The dyes are stable in alcohols and xylene, making them suitable for various microscopy techniques.

Conclusions:

  • NorthernLights secondary antibodies are promising for slide-based cytometry and microscopy due to their photostability, brightness, and affordability.
  • Their spectral compatibility allows use with standard cytometer and microscope excitation sources.
  • These fluorochromes offer enhanced capabilities for multiparametric analysis in fluorescence-based techniques.