Related Experiment Video
Updated: Jun 16, 2026

Uracil-DNA Glycosylase Assay by Matrix-assisted Laser Desorption/Ionization Time-of-flight Mass Spectrometry Analysis
Published on: April 22, 2022
Solid state 2H NMR analysis of furanose ring dynamics in DNA containing uracil
Monica N Kinde-Carson1, Crystal Ferguson, Nathan A Oyler
1Department of Chemistry, University of Nebraska, Lincoln, Nebraska 68588, USA.
None:
DNA damage has been implicated in numerous human diseases, particularly cancer, and the aging process. Single-base lesions, such as uracil, in DNA can be cytotoxic or mutagenic and are recognized by a DNA glycosylase during the process of base excision repair. Increased dynamic properties in lesion-containing DNAs have been suggested to assist recognition and specificity. Deuterium solid-state nuclear magnetic resonance (SSNMR) has been used to directly observe local dynamics of the furanose ring within a uracil:adenine (U:A) base pair and compared to a normal thymine:adenine (T:A) base pair. Quadrupole echo lineshapes,
Related Concept Videos
¹H NMR of Conformationally Flexible Molecules: Temporal Resolution
¹H NMR of Conformationally Flexible Molecules: Variable-Temperature NMR
NMR Spectroscopy of Aromatic Compounds
UV–Vis Spectroscopy: Woodward–Fieser Rules
IR and UV–Vis Spectroscopy of Aldehydes and Ketones
2D NMR: Heteronuclear Single-Quantum Correlation Spectroscopy (HSQC)

