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Updated: Jun 16, 2026
07:26
Quantitative PCR-based Assay to Measure Sonic Hedgehog Signaling in Cellular Model of Ciliogenesis
Published on: January 31, 2025
Increased expression of the hedgehog signaling pathway in pediatric solid malignancies
Takaharu Oue1, Akihiro Yoneda, Shuichiro Uehara
1Division of Pediatric Surgery, Department of Surgery, Osaka University Graduate school of Medicine, Osaka 565-0871, Japan. ooue@pedsurg.med.osaka-u.ac.jp
Journal of Pediatric Surgery
|February 16, 2010
Summary
The hedgehog (Hh) signaling pathway is active in most pediatric cancers, suggesting its role in tumor development. This pathway may influence the differentiation and malignancy of pediatric tumors.
Area of Science:
- Oncology
- Molecular Biology
- Developmental Biology
Background:
- The hedgehog (Hh) signaling pathway is implicated in various cancers.
- Its role in pediatric malignancies is not well-established.
Purpose of the Study:
- To investigate the expression of the Hh signaling pathway in pediatric tumors.
- To clarify the involvement of Hh signaling in pediatric tumorigenesis.
Main Methods:
- Immunohistochemical staining of surgical specimens from 68 pediatric cancer patients.
- Assessed expression of sonic hedgehog (Shh), Patched (Ptch), and Gli1.
Main Results:
- High expression of Shh, Ptch, and Gli1 observed in neuroblastoma, rhabdomyosarcoma, Wilms' tumor, and hepatoblastoma.
- Gli1 expression correlated with differentiation in neuroblastoma and subtype in rhabdomyosarcoma.
Conclusions:
- The Shh-Ptch1-Gli1 pathway is frequently activated in pediatric malignant tumors.
- Hh signaling likely plays a significant role in pediatric cancer differentiation and malignant potential.
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