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A high-performance fluorosensor for pH measurements between 6 and 9.

Aron Hakonen1, Stefan Hulth

  • 1Department of Chemistry, University of Gothenburg, SE-412 96 Göteborg, Sweden. hakonen@chem.gu.se

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Summary
This summary is machine-generated.

A novel ratiometric pH optode using 6,8-dihydroxypyrene-1,3-disulfonic acid (DHPDS) offers high-performance pH sensing. This DHPDS sensor maintains sensitivity across varying ionic strengths, enabling simultaneous pH and ionic strength measurements.

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Area of Science:

  • Analytical Chemistry
  • Materials Science
  • Optical Sensing

Background:

  • Accurate pH monitoring is crucial in various scientific fields.
  • Traditional pH electrodes can be affected by ionic strength.
  • Development of robust optical sensors is an ongoing area of research.

Purpose of the Study:

  • To develop a high-performance ratiometric pH optode.
  • To utilize the fluorophore 6,8-dihydroxypyrene-1,3-disulfonic acid (DHPDS) for pH sensing.
  • To investigate the sensor's performance under varying ionic strengths.

Main Methods:

  • Fabrication of a ratiometric pH optode using DHPDS.
  • Characterization of dual excitation/dual emission properties.
  • Ratiometric signal normalization for pH determination.
  • Evaluation of sensor precision and comparison with a commercial pH electrode.
  • Assessment of the effect of ionic strength on sensor performance.

Main Results:

  • The DHPDS-based sensor exhibited an exponential response to pH changes.
  • A linear correlation was observed between log(R(F1,F2)) and pH in the 6-9 range (R(2)=0.9936).
  • The sensor achieved high precision (0.0037 pH units between calibration points).
  • Unlike conventional electrodes, the DHPDS sensor maintained sensitivity across a wide ionic strength range (10-700 mM).

Conclusions:

  • Immobilized DHPDS enables high-performance, ratiometric pH measurements.
  • The sensor demonstrates superior stability against ionic strength variations.
  • The unique properties of DHPDS allow for simultaneous measurement of pH and ionic strength.