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Updated: Jun 16, 2026

Candidate Gene Testing in Clinical Cohort Studies with Multiplexed Genotyping and Mass Spectrometry
Published on: June 21, 2018
Simultaneous genotyping of microsatellite variations in HMOX1 and UGT1A1 genes using multicolored capillary
Alena Jiraskova1, Martin Lenicek, Libor Vitek
1Department of Internal Medicine, Charles University in Prague, Czech Republic. alena@biomed.cas.cz
Objectives:
Our aim was to establish a reliable, rapid, and inexpensive method for the simultaneous genotyping of the HMOX-1 (heme oxygenase-1) and UGT1A1 (bilirubin UDP-glucuronosyltransferase) gene promoter variations.
Results:
The HMOX1 (GT)(n) and UGT1A1 (TA)(n) gene promoter variations were determined by fragment analysis using a single duplex PCR, with different fluorescent dye-labeled primers; followed by multicolored capillary electrophoresis.
Conclusion:
This novel method provides simultaneous genotyping of key tandem repeat variations in the HMOX1 and UGT1A1 promoters.

