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Related Concept Videos

Cell Specific Gene Expression01:58

Cell Specific Gene Expression

Multicellular organisms contain a variety of structurally and functionally distinct cell types, but the DNA in all the cells originated from the same parent cells. The differences in the cells can be attributed to the differential gene expression. Liver cells, whose functions include detoxification of blood, production of bile to metabolize fats, and synthesis of proteins essential for metabolism, must express a specific set of genes to perform their functions. Gene expression also varies with...
ABC Transporters: Exporter01:31

ABC Transporters: Exporter

ATP-binding cassette or ABC transporter is the largest superfamily of integral membrane proteins. The transporters have transmembrane-binding domains (TMDs) and nucleotide-binding domains (NBDs). The TMDs are specific to their substrates, whereas the NBDs are similar to engines that complete ATP hydrolysis to complete the substrate transport. They can be full transporters consisting of two TMDs and NBDs, half transporters with one TMD and NBD, while some encoded with a single TMD or NBD are...
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Overview
Antibodies, also known as immunoglobulins (Ig), are essential players of the adaptive immune system. These antigen-binding proteins are produced by B cells and make up 20 percent of the total blood plasma by weight. In mammals, antibodies fall into five different classes, which each elicits a different biological response upon antigen binding.
The Y-Shaped Structure of Antibodies Consists of Four Polypeptide Chains
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Antibody Structure01:10

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Antibodies consist of four polypeptide chains: two identical heavy...
ABC Transporters: Importer01:27

ABC Transporters: Importer

ATP-binding cassette or ABC transporters are a class of ATP-driven pumps that hydrolyze ATP to move solutes across the membrane. They can be grouped into importers and exporters. While exporters are present in all domains of life, importers exist only in bacteria and some plants.
In bacteria, based on the number of transmembrane helices and the chemical nature of their substrates, the ABC importers can be divided into three types:
The Central Dogma01:25

The Central Dogma

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Related Experiment Video

Updated: Jun 16, 2026

Methods to Discover Alternative Promoter Usage and Transcriptional Regulation of Murine Bcrp1
11:02

Methods to Discover Alternative Promoter Usage and Transcriptional Regulation of Murine Bcrp1

Published on: May 27, 2016

Canine ABCB4: Tissue expression and cDNA structure.

Erick S Spencer1, Jonathan Minch, Kevin K Lahmers

  • 1Department of Veterinary Clinical Sciences, College of Veterinary Medicine, Washington State University, Pullman, WA 99164-6610, USA.

Research in Veterinary Science
|February 16, 2010
PubMed
Summary
This summary is machine-generated.

Researchers elucidated the canine ABCB4 gene, finding it 89% identical to human ABCB4. Canine liver ABCB4 expression suggests a role in biliary function, similar to its human counterpart.

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Area of Science:

  • Molecular biology
  • Genetics
  • Biochemistry

Background:

  • The ABCB gene subfamily encodes ATP-binding cassette transporters crucial for molecule transport.
  • ABCB4 in humans is vital for biliary lipid homeostasis, with its dysfunction linked to cholestatic syndromes.

Purpose of the Study:

  • To investigate the canine ABCB4 gene due to its known role in human biliary function.
  • To elucidate the full cDNA sequence and determine mRNA and protein expression of canine ABCB4.

Main Methods:

  • Full cDNA sequencing of canine ABCB4.
  • Analysis of mRNA and protein expression levels in canine tissues.

Main Results:

  • The canine ABCB4 gene comprises 3804 nucleotides across 26 exons.
  • Canine ABCB4 exhibits 89% sequence identity to human ABCB4.
  • ABCB4 expression was detected in canine liver tissue.

Conclusions:

  • Canine ABCB4 shares significant sequence homology with its human ortholog.
  • Detected expression in canine liver supports a role in biliary function, mirroring human ABCB4.
  • The function of canine ABCB4 in brain tissue requires further investigation.