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Updated: Jun 16, 2026

Expression of Exogenous Antigens in the Mycobacterium bovis BCG Vaccine via Non-genetic Surface Decoration with the Avidin-biotin System
Published on: January 31, 2018
The secretome of a recombinant BCG substrain reveals differences in hypothetical proteins
Mauricio Rodríguez-Alvarez1, Iliana D Palomec-Nava, Guillermo Mendoza-Hernández
1Programa de Inmunología Molecular Microbiana, Departamento de Microbiología y Parasitología, Facultad de Medicina, Universidad Nacional Autónoma de México (UNAM), Edificio de Investigación, 4 degrees piso, Av. Universidad #3000, Coyoacán, 04510 México, D.F., Mexico.
Abstract:
Tuberculosis remains a major human health problem worldwide, and strategies for its prevention include the generation and characterization of new recombinant vaccines containing immunodominant antigens from Mycobacterium tuberculosis. By comparing the secretomes of wild-type Mycobacterium bovis and a PstS1-recombinant M. bovis BCG vaccine substrain (rBCG38), we identified six conserved hypothetical proteins (BCG2696, BCG1674, BCG0372, BCG0427, BCG2436c, and BCG3053) that are differentially expressed. Our findings will aid in the identification of highly immunogenic proteins present in rBCG.
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