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Related Experiment Video

Updated: Jun 16, 2026

Native Polyacrylamide Gel Electrophoresis Immunoblot Analysis of Endogenous IRF5 Dimerization

Native Polyacrylamide Gel Electrophoresis Immunoblot Analysis of Endogenous IRF5 Dimerization

Published on: October 6, 2019

ISG15 and immune diseases.

Young Joo Jeon¹, Hee Min Yoo, Chin Ha Chung

¹School of Biological Sciences, Seoul National University, Seoul 151-742, Republic of Korea.

Biochimica Et Biophysica Acta

|February 16, 2010

Summary

Interferon-stimulated gene 15 (ISG15) protein conjugation, or ISGylation, plays a role in innate immunity. This study explores ISG15

Area of Science:

Immunology
Molecular Biology
Biochemistry

Background:

ISG15 is a ubiquitin-like protein conjugated to target proteins via a unique enzyme cascade.
ISGylation enzymes are induced by type I interferons (IFNs) and other immune stimuli.
ISG15 conjugation is implicated in type I IFN-mediated innate immune responses.

Purpose of the Study:

To explore the biological function of ISG15 and its modification of target proteins.
To investigate the role of ISG15 in immune diseases.

Main Methods:

Mass spectrometry to identify ISG15-conjugated proteins.
Analysis of ISG15 and its conjugates in the context of innate immunity and immune diseases.

Main Results:

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Hundreds of candidate ISG15-conjugated proteins identified, including IFN-induced proteins and signaling regulators.
ISG15 conjugates are involved in type I IFN-mediated innate immune responses.
Recent progress in understanding ISG15's biological function and its implication in immune diseases.

Conclusions:

ISG15 conjugation is a crucial post-translational modification in innate immunity.
Further research into ISG15's function and reversible modification is needed to understand its role in immune diseases.