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Related Experiment Videos

Odorant-evoked potassium changes in the frog olfactory epithelium.

A Khayari1, F Math, D Trotier

  • 1Laboratoire de physiologie générale II, URA 1293 CNRS, Université de Nancy-I, Vandoeuvre-Les-Nancy, France.

Brain Research
|January 18, 1991
PubMed
Summary

Frog olfactory epithelia were studied using electroolfactogram (EOG) and extracellular potassium activity (aK) measurements. Findings suggest K+ ion accumulation in the olfactory epithelium is linked to odorant receptor activation and EOG generation.

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Area of Science:

  • Neuroscience
  • Olfactory receptor mechanisms
  • Sensory physiology

Background:

  • The electroolfactogram (EOG) measures olfactory receptor cell responses to odorants.
  • Extracellular potassium activity (aK) changes are associated with neuronal activity.

Purpose of the Study:

  • To investigate odorant-evoked changes in extracellular potassium activity (aK) within the frog olfactory epithelium.
  • To correlate aK dynamics with electroolfactogram (EOG) signals.
  • To elucidate the role of potassium ions in olfactory signaling.

Main Methods:

  • In vivo electroolfactogram (EOG) recordings in frog olfactory epithelia.
  • Extracellular potassium activity (aK) measurements using depth profiling.
  • Olfactory stimulation with butanol vapors.

Related Experiment Videos

  • Application of ouabain to assess ion pumping mechanisms.

    • Main Results:

    • Odorant stimulation with butanol evoked an increase in aK in the mucus and proximal epithelium, correlating with EOG amplitude.
    • Complex aK waveforms, including an initial decrease, were observed in deeper epithelial regions.
    • Ouabain suppressed the aK decrease, indicating an active potassium pumping mechanism.

    Conclusions:

    • Extracellular potassium accumulation is a key event following neuroreceptor activation in the olfactory epithelium.
    • These potassium dynamics are closely related to the generation of the electroolfactogram (EOG).
    • Evidence suggests an active ion transport system within the deeper olfactory epithelium.