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Related Experiment Video

Updated: Jun 16, 2026

Metal-Limited Growth of Neisseria gonorrhoeae for Characterization of Metal-Responsive Genes and Metal Acquisition from Host Ligands

Metal-Limited Growth of Neisseria gonorrhoeae for Characterization of Metal-Responsive Genes and Metal Acquisition from Host Ligands

Published on: March 4, 2020

The lactoferrin receptor complex in Gram negative bacteria.

Amanda J Beddek¹, Anthony B Schryvers

¹Department of Microbiology and Infectious Diseases, University of Calgary, Rm G503A Health Sciences Center, 3330 Hospital Drive N.W., Calgary, AB, Canada T2N 4N1.

Biometals : an International Journal on the Role of Metal Ions in Biology, Biochemistry, and Medicine

|February 16, 2010

Summary

This summary is machine-generated.

Bacteria use lactoferrin receptors to obtain iron in mammals. These receptors, essential for bacterial survival, are potential vaccine targets against respiratory and genitourinary infections.

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Area of Science:

Microbiology
Immunology
Biochemistry

Background:

Bacteria in mammalian respiratory and genitourinary tracts face iron scarcity.
Host proteins like transferrin and lactoferrin bind available iron.
Lactoferrin, abundant at inflammation sites, yields antimicrobial lactoferricin.

Purpose of the Study:

To investigate the role of lactoferrin receptors in Neisseriaceae and Moraxellaceae bacterial survival.
To identify potential vaccine targets based on bacterial iron acquisition mechanisms.

Main Methods:

Characterization of bacterial surface receptors for host lactoferrin.
Analysis of the function of lactoferrin binding protein A (LbpA) and lactoferrin binding protein B (LbpB).
Assessment of LbpB's role in protecting bacteria from lactoferricin.

Main Results:

Neisseriaceae and Moraxellaceae utilize specific surface receptors to bind and extract iron from lactoferrin.
LbpA is crucial for growth using lactoferrin as an iron source; LbpB plays a facilitating role.
LbpB's negatively charged residues may protect bacteria from lactoferricin's antimicrobial effects.

Conclusions:

Bacterial lactoferrin receptors are vital for survival in iron-limited host environments.
These receptors represent promising targets for vaccine development against pathogenic bacteria.