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Evaluation of T Follicular Helper Cells and Germinal Center Response During Influenza A Virus Infection in Mice
Published on: June 27, 2020
TXNIP regulates germinal center generation by suppressing BCL-6 expression
Yan Shao1, Sang Yong Kim, Daesung Shin
1Cell Therapy Research Center, Korea Research Institute of Bioscience and Biotechnology, Yusong, Taejon 305-333, Republic of Korea.
Immunology Letters
|February 17, 2010
Summary
Thioredoxin interacting protein (TXNIP) suppresses BCL-6 expression, impacting germinal center (GC) formation in B cells. TXNIP deficiency leads to increased GC B cells and reduced antibody production.
Area of Science:
- Immunology
- Cell Biology
- Cancer Research
Background:
- The precise mechanism of germinal center (GC) formation in B cell lymphomagenesis remains unclear.
- Thioredoxin interacting protein (TXNIP), a tumor suppressor, regulates stress responses, redox balance, and cell proliferation.
- TXNIP is also known as vitamin D3 up-regulated protein 1.
Purpose of the Study:
- To investigate the role of TXNIP in the formation of germinal centers (GCs) in peripheral lymphoid organs.
- To elucidate the relationship between TXNIP expression and BCL-6, a key regulator in GC B cells.
Main Methods:
- Comparative analysis of GC formation in wild-type (WT) mice and Txnip(-/-) mice after immunization.
- Assessment of BCL-6 expression, GC B cell percentage, antibody production, and plasma cell numbers.
- Evaluation of Ki-67 expression as a marker of cell proliferation in splenic tissues.
- In vitro studies on TXNIP overexpression and deficiency effects on BCL-6 expression.
Main Results:
- Txnip(-/-) mice showed elevated BCL-6 expression and a higher percentage of GC B cells compared to WT mice.
- Antibody production and plasma cell numbers were reduced in Txnip(-/-) mice.
- A larger population of Ki-67 positive cells was observed in the spleens of Txnip(-/-) mice.
- TXNIP overexpression decreased BCL-6 expression, while TXNIP deficiency increased it.
Conclusions:
- TXNIP plays a significant role in regulating germinal center formation following immunization.
- TXNIP functions, in part, by suppressing the expression of BCL-6.
- These findings suggest TXNIP as a potential target for modulating GC reactions and B cell lymphomagenesis.
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