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Published on: July 27, 2018
Complete nucleic acid sequence of Penaeus monodon densovirus (PmDNV) from India
Muhammed P Safeena1, Anuj Tyagi, Praveen Rai
1Department of Fishery Microbiology, Karnataka Veterinary, Animal and Fisheries Sciences, University, College of Fisheries, Mangalore-575 002, India.
Virus Research
|February 17, 2010
Summary
The complete genome of the Indian Penaeus monodon densovirus (PmDNV) was sequenced and analyzed. This shrimp virus shows the highest genetic similarity to the Thai PmDNV strain.
Area of Science:
- * Virology
- * Molecular Biology
- * Genetics
Background:
- * Penaeus monodon densovirus (PmDNV) is a significant pathogen affecting shrimp aquaculture.
- * Understanding viral genetic diversity is crucial for disease management and prevention.
Purpose of the Study:
- * To characterize the complete genome sequence of PmDNV from India.
- * To compare the Indian PmDNV isolate with other known strains and related parvoviruses.
Main Methods:
- * Whole-genome sequencing of the Indian PmDNV isolate.
- * Bioinformatic analysis of open reading frames (ORFs) and protein sequences (NS1, NS2, VP).
- * Comparative analysis and phylogenetic analysis with related parvoviruses.
Main Results:
- * The complete genome of Indian PmDNV is 6310 bp, containing three ORFs.
- * Highest nucleotide similarity (88%) was found with the Thai PmDNV strain.
- * Phylogenetic analysis indicated closer relatedness to Thai PmDNV than other parvoviruses.
Conclusions:
- * The Indian PmDNV isolate shares significant genetic similarity with the Thai strain.
- * This finding aids in understanding the geographic distribution and evolution of PmDNV.
- * Further research can inform strategies for controlling PmDNV outbreaks in shrimp populations.
