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Published on: August 4, 2023
Proteomics of microparticles after deep venous thrombosis
Eduardo Ramacciotti1, Angela E Hawley, Shirley K Wrobleski
1Jobst Vascular Research Laboratory, Section of Vascular Surgery, Cardiovascular Center, Department of Surgery, University of Michigan, Ann Arbor, MI, USA. erama@med.umich.edu
Microparticles (MPs) from deep venous thrombosis (DVT) patients showed elevated Galectin-3 Binding Protein and Alpha-2 macroglobulin. These proteins may play a role in DVT pathogenesis and inflammation.
Area of Science:
- Proteomics
- Biochemistry
- Thrombosis Research
Background:
- Microparticles (MPs) are cell-derived vesicles linked to thrombosis and inflammation.
- MPs express proteins reflecting their cell origin, potentially indicating disease states.
Purpose of the Study:
- To identify proteins differentially expressed on MPs from deep venous thrombosis (DVT) patients.
- To investigate the proteomic profile of MPs in relation to DVT.
Main Methods:
- Plasma samples from DVT patients, patients with leg pain, and healthy controls were analyzed.
- Microparticles were isolated, digested, and analyzed using iTRAQ labeling and 2D LC-MALDI tandem mass spectrometry.
- Proteins were quantified, and differential expression was determined based on iTRAQ ratios and p-values.
Main Results:
- Galectin-3 Binding Protein (Gal3BP) and Alpha-2 macroglobulin (A2M) were significantly elevated in DVT patients.
- Fibrinogen beta and gamma chain precursors were among nine proteins found to be depleted.
Conclusions:
- Elevated Gal3BP and A2M in MPs may contribute to DVT pathogenesis.
- These proteins influence inflammation, cell shedding, and hemostasis.
- Further research is required to elucidate the precise role of these proteins in human venous thrombosis.
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