Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

IgG subclasses in circulating immune complexes with hepatitis B e antigen in chronic hepatitis B.

M Sällberg1, H Norder, G Lindh

  • 1Department of Virology, National Bacteriological Laboratory, Stockholm, Sweden.

Clinical and Experimental Immunology
|April 1, 1991
PubMed
Summary

Complexed antibodies to hepatitis B e antigen (anti-HBe) IgG subclasses were analyzed in HBsAg-positive sera. Complexed anti-HBe IgG1 and IgG4 were frequently detected, potentially indicating seroconversion years in advance.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

ATMP development and pre-GMP environment in academia: a safety net for early cell and gene therapy development and manufacturing.

Immuno-oncology technology·2022
Same author

Designing a therapeutic hepatitis B vaccine to circumvent immune tolerance.

Human vaccines & immunotherapeutics·2019
Same author

Incidence of Hepatitis E Antibodies in Swedish Lung Transplant Recipients.

Transplantation proceedings·2015
Same author

[Molecular epidemiology of hepatitis C in centers of hemodialysis in St. Petersburg].

Zhurnal mikrobiologii, epidemiologii i immunobiologii·2015
Same author

Long-term follow-up of successful hepatitis C virus therapy: waning immune responses and disappearance of liver disease are consistent with cure.

Alimentary pharmacology & therapeutics·2015
Same author

Complete coding regions of the prototypes enterovirus B93 and C95: phylogenetic analyses of the P1 and P3 regions of EV-B and EV-C strains.

Journal of medical virology·2014

Area of Science:

  • Immunology
  • Hepatology
  • Virology

Background:

  • Hepatitis B e antigen (HBeAg) seroconversion is a key indicator of treatment response.
  • Antibody subclass analysis provides deeper insights into immune responses.
  • Understanding anti-HBe IgG subclass dynamics is crucial for monitoring Hepatitis B virus (HBV) infection.

Purpose of the Study:

  • To determine the IgG subclasses of antibodies to HBeAg (anti-HBe) complexed with HBeAg.
  • To investigate the association between complexed anti-HBe IgG subclasses and HBeAg seroconversion.
  • To evaluate the diagnostic potential of complexed anti-HBe IgG subclasses in predicting seroconversion.

Main Methods:

  • Analysis of IgG subclasses of anti-HBe complexed to HBeAg in 126 HBsAg-positive sera.

Related Experiment Videos

  • Utilized monoclonal anti-HBe for capturing HBeAg complexes on microtitre plates.
  • Employed biotinylated monoclonals for detecting IgG subclasses (IgG1-IgG4) within the complexes.

    • Main Results:

    • Complexed anti-HBe IgG was detected in 22/30 HBeAg-positive and 3/HBeAg-negative carrier sera.
    • IgG1 (75%) and IgG4 (67%) were the most common subclasses found complexed to HBeAg.
    • In patients who seroconverted, anti-HBe IgG1 was detected in the HBeAg-positive phase before seroconversion.

    Conclusions:

    • Complexed anti-HBe may be detectable years before seroconversion in conventional assays.
    • Free anti-HBe IgG1 in HBeAg-positive sera may signal impending seroconversion.
    • IgG subclass analysis offers a novel approach to monitoring HBV infection and predicting treatment outcomes.