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Related Concept Videos

Imaging Biological Samples with Optical Microscopy01:18

Imaging Biological Samples with Optical Microscopy

Optical microscopy uses optic principles to provide detailed images of samples. Antonie van Leeuwenhoek designed the first compound optical microscope in the 17th century to visualize blood cells, bacteria, and yeast cells. In 1830, Joseph Jackson Lister created an essentially modern light microscope. The 20th century saw the development of microscopes with enhanced magnification and resolution.
In optical microscopy, the specimen to be viewed is placed on a glass slide and clipped on the stage...

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Related Experiment Video

Updated: Jun 16, 2026

Single Plane Illumination Module and Micro-capillary Approach for a Wide-field Microscope
08:53

Single Plane Illumination Module and Micro-capillary Approach for a Wide-field Microscope

Published on: August 15, 2014

Microcrystallography using single-bounce monocapillary optics.

R E Gillilan1, M J Cook, S W Cornaby

  • 1MacCHESS (Macromolecular Diffraction Facility at CHESS), Ithaca, NY, USA. reg8@cornell.edu

Journal of Synchrotron Radiation
|February 17, 2010
PubMed
Summary
This summary is machine-generated.

A new method using single-bounce glass monocapillary optics offers an effective way to create X-ray microbeams for protein crystallography. This technique improves diffraction data quality for microcrystals, making it a valuable alternative for existing beamlines.

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Related Experiment Videos

Last Updated: Jun 16, 2026

Single Plane Illumination Module and Micro-capillary Approach for a Wide-field Microscope
08:53

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Published on: August 15, 2014

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09:35

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Published on: July 21, 2017

A Sample Preparation Pipeline for Microcrystals at the VMXm Beamline
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A Sample Preparation Pipeline for Microcrystals at the VMXm Beamline

Published on: June 17, 2021

Area of Science:

  • Protein crystallography
  • Materials science
  • Optics

Background:

  • X-ray microbeams are crucial for analyzing small and challenging protein samples.
  • Current methods often use Kirkpatrick and Baez mirrors, apertures, and scatter guards to generate microbeams.
  • Existing synchrotron beamlines are increasingly equipped for microcrystallography.

Purpose of the Study:

  • To present a simple and robust alternative for generating X-ray microbeams using single-bounce glass monocapillary optics.
  • To demonstrate the effectiveness of monocapillary optics for protein microcrystallography.
  • To provide a practical solution for retrofitting existing beamlines.

Main Methods:

  • A single-bounce glass monocapillary optic design is presented.
  • Considerations for capillary design, mounting, and implementation on beamlines are discussed.
  • Parasitic scattering and optic deformation are minimized through a specialized mounting design.

Main Results:

  • The capillary-focused beam significantly improves diffraction data statistics for microcrystals compared to conventional beams.
  • The annular beam profile produced by the capillary optic does not negatively impact data quality.
  • New protein structures have been solved using this microbeam technology.

Conclusions:

  • Single-bounce monocapillary optics provide an attractive and effective alternative for generating X-ray microbeams.
  • This technology can be readily implemented to upgrade existing synchrotron beamlines for microcrystallography.
  • The method enhances data quality and enables the study of challenging microcrystalline samples.