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Related Experiment Video

Updated: Jun 16, 2026

Mechanism of Regulation of Adipocyte Numbers in Adult Organisms Through Differentiation and Apoptosis Homeostasis
08:34

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Published on: June 3, 2016

C/EBPalpha regulates SIRT1 expression during adipogenesis.

Qihuang Jin1, Fang Zhang, Tingting Yan

  • 1Key Laboratory of Nutrition and Metabolism, Institute for Nutritional Sciences, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 200031, China.

Cell Research
|February 17, 2010
PubMed
Summary
This summary is machine-generated.

CCAAT/enhancer-binding protein alpha (C/EBPalpha) directly regulates Silent information regulator 1 (SIRT1) expression during adipogenesis. This study elucidates the mechanism by which C/EBPalpha controls SIRT1 levels in differentiating cells.

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Last Updated: Jun 16, 2026

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07:22

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Published on: March 28, 2013

Area of Science:

  • Molecular Biology
  • Cell Biology
  • Biochemistry

Background:

  • Silent information regulator 1 (SIRT1) is crucial for adipogenesis.
  • The regulatory mechanisms of SIRT1 during adipocyte differentiation are not well understood.

Purpose of the Study:

  • To investigate how SIRT1 is regulated during adipogenesis.
  • To identify the role of CCAAT/enhancer-binding protein alpha (C/EBPalpha) in SIRT1 regulation.

Main Methods:

  • Analysis of SIRT1 and C/EBPalpha expression during adipocyte differentiation.
  • Reporter assays to assess SIRT1 promoter activity.
  • Western blotting and qRT-PCR to measure protein and mRNA levels.
  • Chromatin immunoprecipitation and gel shift assays to confirm DNA binding.

Main Results:

  • Both SIRT1 protein and mRNA levels increased with C/EBPalpha during adipocyte differentiation.
  • C/EBPalpha directly activated the SIRT1 promoter.
  • C/EBPalpha upregulated SIRT1 expression in a p53-independent manner.
  • C/EBPalpha was confirmed to bind directly to the SIRT1 promoter.

Conclusions:

  • CCAAT/enhancer-binding protein alpha (C/EBPalpha) directly binds to the SIRT1 promoter.
  • C/EBPalpha acts as a transcriptional regulator of SIRT1 during adipogenesis.