Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Liver Regeneration01:24

Liver Regeneration

The liver is an important organ in vertebrates that plays an essential role in metabolism. It is also responsible for storing and redistributing nutrients such as carbohydrates, fats, and vitamins in the body. Additionally, the liver releases bile salts which are critical for digesting food and eliminating toxic metabolites from the body.
Cells of Liver
The liver comprises four major types of cells— hepatocytes, stellate, Kupffer, and sinusoidal endothelial cells. The hepatocytes are large...
Cirrhosis II: Pathophysiology01:24

Cirrhosis II: Pathophysiology

Cirrhosis is a progressive chronic liver injury caused by prolonged inflammation, excessive fibrotic remodeling, and impaired regeneration. Over time, repeated hepatic insults disrupt the liver’s architecture and function, leading to reduced blood flow, impaired bile drainage, and diminished metabolic capacity.Pathophysiology of cirrhosisCirrhosis arises from three main responses to chronic liver damage: inflammation, immune activation, and hepatocyte death. These processes lead to structural...
Pharmacokinetics in Geriatric Patients: Effect of Age on Drug Metabolism01:18

Pharmacokinetics in Geriatric Patients: Effect of Age on Drug Metabolism

Geriatric patients show significant variation in how their bodies process medications, which can change how effective and safe treatments are. The liver is the primary organ where drug metabolism occurs, involving two main types of chemical reactions: phase I and II. Phase I metabolism is driven by the cytochrome P450 enzyme system, which includes key types such as CYP3A, CYP2D6, and CYP2C9. Research indicates that while aging doesn't notably alter the levels or activity of these enzymes, it...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Fusion <i>DNAJB1::PRKACA</i> in Non-Fibrolamellar Cancer Cases. Reply to Arif et al. Global Re-Analysis Confirms Absence of the <i>DNAJB1::PRKACA</i> Fusion in Hepatoblastoma. Comment on "Fleifil et al. DNAJB1-PKAc Kinase Is Expressed in Young Patients with Pediatric Liver Cancers and Enhances Carcinogenic Pathways. <i>Cancers</i> 2025, <i>17</i>, 83".

Cancers·2026
Same author

Investigating the oncogenic role of aberrant EZH2 in hepatoblastoma.

Scientific reports·2026
Same author

Elevated Levels of Active GSK3β in the Blood of Patients with Myotonic Dystrophy Type 1 Correlate with Muscle Weakness.

International journal of molecular sciences·2025
Same author

Sensory integration deficits in Parkinson's disease with freezing of gait: cortical network dynamics and paradoxical dopaminergic modulation.

Journal of neurology·2025
Same author

CircSipa1l1 modulates melanoma cell differentiation by activating the IGF2BP1-ARHGDIB axis and ERK signaling pathway.

Journal of translational medicine·2025
Same author

Genome-wide CRISPR screens identify critical targets to enhance CAR-NK cell antitumor potency.

Cancer cell·2025

Related Experiment Video

Updated: Jun 16, 2026

Isolation of Intermediate Filament Proteins from Multiple Mouse Tissues to Study Aging-associated Post-translational Modifications
09:29

Isolation of Intermediate Filament Proteins from Multiple Mouse Tissues to Study Aging-associated Post-translational Modifications

Published on: May 18, 2017

GSK3beta and aging liver.

Jingling Jin1, Guo-Li Wang, Lubov Timchenko

  • 1Huffington Center on Aging and Department of Pathology, Baylor College of Medicine, Houston, TX 77030, USA.

Aging
|February 17, 2010
PubMed
Summary

Aging reduces tissue regeneration by epigenetically silencing cell cycle genes in the liver. New research identifies upstream pathways involving growth hormone, GSK3beta, and cyclin D3, offering insights into aging mechanisms.

Keywords:
C/EBPGSK3agingcyclin D3liverproliferation

Related Experiment Videos

Last Updated: Jun 16, 2026

Isolation of Intermediate Filament Proteins from Multiple Mouse Tissues to Study Aging-associated Post-translational Modifications
09:29

Isolation of Intermediate Filament Proteins from Multiple Mouse Tissues to Study Aging-associated Post-translational Modifications

Published on: May 18, 2017

Area of Science:

  • Gerontology
  • Molecular Biology
  • Epigenetics

Background:

  • Tissue regenerative capacity declines with age, a key aging characteristic.
  • Liver regeneration is impaired in aged mice due to epigenetic silencing of cell cycle proteins.
  • The C/EBPalpha-HDAC1-Brm complex silences genes like c-myc and FoxM1B in aged livers.

Purpose of the Study:

  • To investigate the upstream mechanisms driving age-associated epigenetic silencing in liver regeneration.
  • To explore the role of growth hormone (GH), GSK3beta, and cyclin D3 in regulating the C/EBPalpha-HDAC1-Brm complex.
  • To examine if these pathways are altered in other aging tissues.

Main Methods:

  • Review of recent studies on epigenetic regulation of liver proliferation in aging mice.
  • Analysis of signal transduction pathways involving GH, GSK3beta, and cyclin D3.
  • Comparison of pathway alterations in liver, lung, brain, and adipose tissues.

Main Results:

  • Aging causes epigenetic silencing of cell cycle genes in the liver via the C/EBPalpha-HDAC1-Brm complex.
  • Novel upstream pathways involving GH, GSK3beta, and cyclin D3 regulate this complex.
  • These GH-GSK3beta-cyclin D3 pathway alterations are also observed in lung, brain, and adipose tissues with age.

Conclusions:

  • Age-related decline in liver regeneration is linked to epigenetic silencing mediated by the C/EBPalpha-HDAC1-Brm complex.
  • The GH-GSK3beta-cyclin D3 pathway represents a critical upstream regulator of this silencing.
  • Dysregulation of this pathway may contribute to the aging phenotype in multiple tissues.