Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Notch Signaling Pathway03:14

Notch Signaling Pathway

The Notch signaling pathway is a major intracellular signaling pathway that is highly conserved over a broad spectrum of metazoan species. It stands unique from other intracellular signaling mechanisms in animals because notch protein itself acts as the receptor as well as the primary signaling molecule.
The Notch gene came into the limelight in 1914 after the discovery that its mutation in Drosophila melanogaster leads to a serrated (or "notched") wing margin phenotype. It was not until 1985...
IP3/DAG Signaling Pathway01:11

IP3/DAG Signaling Pathway

Membrane lipids such as phosphatidylinositol (PI) are precursors for several membrane-bound and soluble second messengers. Specific kinases phosphorylate PI and produce phosphorylated inositol phospholipids. One such inositol phospholipids are the  phosphatidylinositol-4,5 bisphosphate [PI(4,5)P2], present in the inner half of the lipid bilayer. Upon ligand binding, GPCR stimulates Gq proteins to turn on phospholipase Cꞵ. Activated phospholipase Cꞵ cleaves PI(4,5)P2 and produces two-second...
Determination01:51

Determination

During embryogenesis, cells become progressively committed to different fates through a two-step process: specification followed by determination. Specification is demonstrated by removing a segment of an early embryo, “neutrally” culturing the tissue in vitro—for example, in a petri dish with simple medium—and then observing the derivatives. If the cultured region gives rise to cell types that it would normally generate in the embryo, this means that it is specified. In contrast, determination...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

TNAP-mediated hydrolysis of phosphorylated enamel matrix proteins drives local phosphate generation and enamel mineralization.

The Journal of biological chemistry·2026
Same author

Solute carrier family 9 member A5 regulated by TGF-β is necessary for dental enamel formation.

Journal of oral biosciences·2026
Same author

Developmental vulnerability to fluoride toxicity: enamel and clearance differences in adolescent versus mature mice.

Archives of toxicology·2025
Same author

Fibroblast growth factor 2 promotes matrix vesicle-mediated mineralization of human umbilical cord perivascular cells by regulating phosphate metabolism.

Journal of oral biosciences·2025
Same author

Direct and indirect effects of transforming growth factor-beta on osteoclast-mediated bone remodeling using a new in vitro bone matrix model.

JBMR plus·2025
Same author

Citrate Transporter Expression and Localization: The <i>Slc13a5</i><sup>Flag</sup> Mouse Model.

International journal of molecular sciences·2025
Same journal

Metformin suppresses periapical bone resorption in a mouse model of apical periodontitis in vivo and inhibits RANKL-induced osteoclastogenesis in vitro.

Journal of oral biosciences·2026
Same journal

Zinc transporter ZIP13 in mesenchymal tissues: From intracellular metal distribution to systemic homeostasis.

Journal of oral biosciences·2026
Same journal

Relationship between ear canal deformation and mandibular condyle movement using an earplug-type sensor.

Journal of oral biosciences·2026
Same journal

Erratum to "Recent advances in the treatment of oral ulcerative mucositis from clinical and basic perspectives" [J Oral Biosci 66 (3) (September 2024) 504-510].

Journal of oral biosciences·2026
Same journal

Pathophysiology of orofacial neuropathic pain: A narrative review on the multi-level cascade of neuro-glial plasticity.

Journal of oral biosciences·2026
Same journal

Orthodontic force induces local bone resorption in mild-type hypophosphatasia mouse model.

Journal of oral biosciences·2026
See all related articles

Related Experiment Video

Updated: Jun 16, 2026

Accessing the Cytotoxicity and Cell Response to Biomaterials
09:46

Accessing the Cytotoxicity and Cell Response to Biomaterials

Published on: July 8, 2021

Dentinogenesis and Dentin Sialophosphoprotein (DSPP).

Yasuo Yamakoshi1

  • 1Department of Biologic and Materials Sciences, University of Michigan Dental Research Lab, 1210 Eisenhower Place, Ann Arbor, MI 48108, Tel: 734-975-9358 (Office); Tel: 734-975-9326 (Lab); 734-975-9329 (Fax), yamasan@umich.edu.

Journal of Oral Biosciences
|February 17, 2010
PubMed
Summary
This summary is machine-generated.

Dentin sialophosphoprotein (DSPP) is vital for tooth dentin mineralization. Research characterized DSPP-derived proteins, revealing their structures and functions in porcine teeth, aiding biomineralization understanding.

More Related Videos

Isolation, Characterization and Comparative Differentiation of Human Dental Pulp Stem Cells Derived from Permanent Teeth by Using Two Different Methods
14:52

Isolation, Characterization and Comparative Differentiation of Human Dental Pulp Stem Cells Derived from Permanent Teeth by Using Two Different Methods

Published on: November 24, 2012

Isolation, Propagation, and Prion Protein Expression During Neuronal Differentiation of Human Dental Pulp Stem Cells
11:50

Isolation, Propagation, and Prion Protein Expression During Neuronal Differentiation of Human Dental Pulp Stem Cells

Published on: March 18, 2019

Related Experiment Videos

Last Updated: Jun 16, 2026

Accessing the Cytotoxicity and Cell Response to Biomaterials
09:46

Accessing the Cytotoxicity and Cell Response to Biomaterials

Published on: July 8, 2021

Isolation, Characterization and Comparative Differentiation of Human Dental Pulp Stem Cells Derived from Permanent Teeth by Using Two Different Methods
14:52

Isolation, Characterization and Comparative Differentiation of Human Dental Pulp Stem Cells Derived from Permanent Teeth by Using Two Different Methods

Published on: November 24, 2012

Isolation, Propagation, and Prion Protein Expression During Neuronal Differentiation of Human Dental Pulp Stem Cells
11:50

Isolation, Propagation, and Prion Protein Expression During Neuronal Differentiation of Human Dental Pulp Stem Cells

Published on: March 18, 2019

Area of Science:

  • Biochemistry
  • Molecular Biology
  • Dental Research

Background:

  • Dentin sialophosphoprotein (DSPP) plays a crucial role in tooth dentin mineralization.
  • Understanding DSPP's structure and function is key to controlling dentin biomineralization.

Purpose of the Study:

  • To characterize DSPP-derived proteins from developing porcine teeth.
  • To elucidate the structure and function of DSPP fragments.

Main Methods:

  • Isolation and characterization of DSPP-derived proteins from porcine teeth.
  • Analysis of protein processing by enzymes like BMP-1, MMP-20, and MMP-2.

Main Results:

  • DSPP is processed into dentin sialoprotein (DSP), dentin glycoprotein (DGP), and dentin phosphoprotein (DPP).
  • DSP forms covalent dimers and is a proteoglycan.
  • DGP is a phosphorylated glycoprotein.
  • DPP is a highly phosphorylated, intrinsically disordered protein with length polymorphisms.

Conclusions:

  • The characterization of DSPP fragments provides insights into dentin biomineralization mechanisms.
  • DSPP processing and the properties of its derivatives are critical for proper tooth development.