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Related Experiment Videos

Endodermal origin of yolk-sac-derived teratomas.

H Sobis1, A Verstuyf, M Vandeputte

  • 1Rega Institute for Medical Research, University of Leuven, Belgium.

Development (Cambridge, England)
|January 1, 1991
PubMed
Summary

Teratomas derived from mouse yolk sacs originate from endodermal cells. This is because the paternal X chromosome is inactivated in yolk sac endoderm, unlike in mesoderm or embryos where inactivation is random.

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Area of Science:

  • Developmental Biology
  • Genetics
  • Teratoma Research

Background:

  • Glucose-6-phosphate dehydrogenase (G6PD) is an X-linked enzyme crucial for cellular metabolism.
  • X chromosome inactivation in female mammals leads to mosaicism for X-linked gene expression.
  • Teratomas are tumors with diverse cell types, often arising from pluripotent cells.

Purpose of the Study:

  • To investigate the cellular origin of yolk sac-derived teratomas using G6PD deficiency as a marker.
  • To determine the pattern of X chromosome inactivation in different embryonic and extraembryonic tissues.
  • To elucidate the developmental lineage of teratomas formed from the yolk sac.

Main Methods:

  • Utilized mutant mice deficient in glucose-6-phosphate dehydrogenase (G6PD).
  • Employed crosses between wild-type and G6PD-deficient mice to generate heterozygous and homozygous concepti.
  • Performed histochemical analysis to detect G6PD enzyme activity in embryonic and teratoma tissues.

Main Results:

  • Heterozygous embryos and their derived embryomas exhibited dual G6PD activity, indicating random X inactivation.
  • Teratomas derived from the yolk sac showed distinct G6PD activity patterns based on maternal genotype.
  • Yolk sac-derived teratomas from homozygous deficient mothers were G6PD-negative, while those from wild-type mothers were G6PD-positive.

Conclusions:

  • Yolk sac-derived teratomas originate from endodermal cells.
  • This conclusion is supported by the non-random paternal X chromosome inactivation observed specifically in yolk sac endoderm.
  • Contrasts with random X inactivation in yolk sac mesoderm and the embryo proper.

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