Agrobacterium rhizogenes-mediated transformation of Hypericum tomentosum L and Hypericum tetrapterum Fries
Hedviga Komarovská1, Annalisa Giovannini, Ján Kosutha
1P. J. Safirik University, Faculty of Science, Institute of Biology and Ecology, Mánesova 23, 041 54 KoSice, Slovakia.
Abstract:
This is the first evidence on successful Agrobacterium rhizogenes-mediated genetic transformation of two species from the genus Hypericum, H. tomentosum L. and H. tetrapterum Fries. Hairy root cultures were induced from root segments of both Hypericum species by two agropine wild-type strains of A. rhizogenes, ATCC 15834 and A4. The transgenic character of the hairy root cultures was proved by PCR amplification of the rolABCD genes. In some H. tetrapterum transgenic lines aux genes were detected as well.
Related Concept Videos
Transgenic Plants
The first-ever transgenic plant was a tobacco plant developed in 1983 that showed resistance against the tobacco mosaic virus. Since then, many transgenic plants have been developed and commercialized for improving the agricultural, ornamental, and horticultural value of a crop plant. Transgenic...
Bacterial Transformation
Transgenic Organisms

