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Updated: Jun 16, 2026

Angiogenesis in the Ischemic Rat Lung
Published on: February 8, 2013
[Hydrogen dioxide increases the expression of vascular endothelial growth factor in human bronchiolar epithelium cell
Xiang-bo Shen1, Hai-jin Zhao, Shao-xi Cai
1Department of Respiratory Disease, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China. shenxiangbo333 @126.com
Objective:
To investigate the effect of hydrogen dioxide (H(2)O(2)) on the expression of vascular endothelial growth factor (VEGF) in human bronchiolar epithelial (HBE) cells.
Methods:
MTT assay was used to assess HBE cell viability after exposure to different concentrations of H(2)O(2). VEGF/beta-actin gene fragments were amplified simultaneously by RT-PCR from the total HBE cell RNA, and VEGF protein expression in the cells was detected using ELISA.
Results:
The exposure to 200 micromol/L H(2)O(2) did not obviously affected the cell viability. Compared with those in the control cell, VEGF165/beta-actin and VEGF189/beta-actin ratios were significantly increased in the cells after treatment with 50, 200, and 600 micromol/L H(2)O(2) (P<0.05). The protein expression of VEGF significantly increased after 50 micromol/L H(2)O(2) treatment (P<0.05), but significantly decreased with pretreatment with the PI3K inhibitor Ly294002 (P>0.05).
Conclusion:
Oxidative stress increases the expression of VEGF via a PI3K-dependent pathway in human bronchiolar epithelial cells, which may play an important role in the onset and maintenance of chronic inflammation in asthma.
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