Changes in the mesenteric lymph proteome induced by hemorrhagic shock

Area of Science:

• Physiology
• Biochemistry
• Proteomics

Background:

• Intestinal factors transported by mesenteric lymph contribute to organ failure in hemorrhagic shock.
• The protein composition of shock-conditioned mesenteric lymph is not well-described, hindering understanding of its role.

Purpose of the Study:

• To provide the first comprehensive proteomic description of mesenteric lymph following hemorrhagic shock.
• To identify specific proteins and biological pathways altered by hemorrhagic shock in mesenteric lymph.

Main Methods:

• Mesenteric lymph was collected from Wistar rats subjected to hemorrhagic shock or sham control.
• Proteomic analysis was performed using iTRAQ and liquid chromatography-tandem mass spectrometry.
• Bioinformatics and interactome analysis were used to identify key proteins and pathways.

Main Results:

• A total of 245 proteins were identified in the mesenteric lymph.
• Sixty proteins showed a significant increase in relative abundance in the hemorrhagic shock group.
• Key altered pathways included response to injury and metabolic responses; several highly connected proteins were identified.

Conclusions:

• This study presents the first detailed proteomic profile of mesenteric lymph in hemorrhagic shock.
• Identified proteins and pathways represent potential targets for further investigation and therapeutic strategies.
• Understanding these molecular changes is crucial for addressing distant organ failure in shock.