Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Novel human PDGFA gene transcripts derived by alternative mRNA splicing.

A Sánchez1, C N Chesterman, M J Sleigh

  • 1CSIRO, Division of Biomolecular Engineering, North Ryde, NSW, Australia.

Gene
|February 15, 1991
PubMed
Summary

Researchers used polymerase chain reaction (PCR) to study platelet-derived growth factor A chain (PDGFA) gene expression. They discovered novel RNA variants lacking exon 2, suggesting a new post-transcriptional regulation mechanism for PDGFA.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Effects of kappa- and mu-opioid receptor agonists on Ca2+ channels in neuroblastoma cells: involvement of the orphan opioid receptor.

European journal of pharmacology·1999
Same author

Mutational analysis of the putative fusion domain of Ebola virus glycoprotein.

Journal of virology·1999
Same author

Activities of new antimicrobial agents (trovafloxacin, moxifloxacin, sanfetrinem, and quinupristin-dalfopristin) against Bacteroides fragilis group: comparison with the activities of 14 other agents.

Antimicrobial agents and chemotherapy·1999
Same author

Hyperenhancing focal liver lesions: differential diagnosis with helical CT.

AJR. American journal of roentgenology·1999
Same author

Formulation strategies for the stabilization of tetanus toxoid in poly(lactide-co-glycolide) microspheres.

International journal of pharmaceutics·1999
Same author

Post-weaning multisystemic wasting syndrome in Spain.

The Veterinary record·1999

Area of Science:

  • Molecular Biology
  • Genetics
  • Cell Biology

Background:

  • Platelet-derived growth factor A chain (PDGFA) plays a crucial role in cell growth and differentiation.
  • Previous studies identified two main PDGFA transcripts differing in exon 6.
  • Understanding PDGFA gene expression regulation is vital for comprehending cellular processes.

Purpose of the Study:

  • To investigate PDGFA gene expression in human cell lines and umbilical vein cells.
  • To identify and characterize novel PDGFA transcripts.
  • To explore potential post-transcriptional mechanisms regulating PDGFA.

Main Methods:

  • Polymerase chain reaction (PCR) was employed to amplify PDGFA sequences from mRNA.
  • Cloning and sequencing were used to analyze the amplified RNA products.

Related Experiment Videos

  • Analysis was performed on mRNA from human osteosarcoma (U-2OS), human glioma (U-343), and human umbilical vein cells.

    • Main Results:

    • The study confirmed the presence of previously reported PDGFA transcripts.
    • Novel RNA species lacking exon 2 were identified in all cell types examined.
    • These exon 2-deficient transcripts were found at variable levels across different cell types.

    Conclusions:

    • A novel splicing pattern resulting in exon 2-deficient PDGFA mRNA was discovered.
    • This alternative splicing generates truncated, non-functional PDGFA polypeptides.
    • This finding suggests a significant post-transcriptional mechanism for modulating PDGFA gene expression.