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Updated: Jun 16, 2026

Fabrication of Refractive-index-matched Devices for Biomedical Microfluidics
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Self-reference quantitative phase microscopy for microfluidic devices.

Jaeduck Jang1, Chae Yun Bae, Je-Kyun Park

  • 1Bio Imaging & Signal Processing Laboratory, Department of Bio and Brain Engineering,Korea Advanced Institute of Science and Technology, Daejeon 305-701, Korea.

Optics Letters
|February 18, 2010
PubMed
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This study introduces a quantitative phase microscopy technique for microfluidic devices. The simple self-referencing interferometry method enhances imaging depth-of-field for clearer cell and material analysis.

Area of Science:

  • Biomedical Optics
  • Microfluidics
  • Phase Contrast Microscopy

Background:

  • Microfluidic devices enable precise control and analysis of small volumes.
  • Quantitative phase microscopy (QPM) provides label-free imaging of transparent specimens.
  • Traditional QPM methods often face limitations in depth-of-field and system complexity.

Purpose of the Study:

  • To develop a simple, self-referencing quantitative phase microscopy system for microfluidic devices.
  • To achieve extended depth-of-field imaging without mechanical adjustments.
  • To enable high signal-to-noise ratio imaging adaptable to Hilbert phase microscopy.

Main Methods:

  • Utilized a self-referencing interferometry approach within a Michelson interferometer setup.
  • Implemented extended depth-of-field optics to eliminate the need for objective lens or specimen movement.

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  • Designed the system for simultaneous acquisition of double interferograms for enhanced signal-to-noise ratio.

    • Main Results:

    • Demonstrated successful quantitative phase measurement with an increased depth-of-field in microchannels.
    • Verified the system's performance using polymer beads, patterned poly(dimethylsiloxane) (PDMS), and embryo cells.
    • Showcased the system's adaptability to Hilbert phase microscopy for improved image quality.

    Conclusions:

    • The developed quantitative phase microscopy system offers a straightforward and effective method for microfluidic applications.
    • The extended depth-of-field and high signal-to-noise ratio capabilities facilitate detailed analysis of microscale biological and material samples.
    • This technique provides a valuable tool for label-free imaging and characterization within microfluidic environments.