Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Elevated cyclic AMP concentration in streptomycin-dependent Escherichia coli.

W J Polglase, D Iwacha, M Thomson

    Journal of Bacteriology
    |January 1, 1978
    PubMed
    Summary

    Streptomycin-dependent Escherichia coli cultures exhibit relaxed catabolite repression and elevated cyclic AMP levels when grown in glucose-salts medium. This suggests a link between nutrient availability and cellular signaling pathways in bacteria.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Search for the Y(2175) in the Photoproduction Cross Section Measurement of γp→ϕπ^{+}π^{-}p at GlueX.

    Physical review letters·2026
    Same author

    Search for an Excess of Electron Neutrino Interactions in MicroBooNE Using Multiple Final-State Topologies.

    Physical review letters·2022
    Same author

    An ESPGHAN Position Paper on the Use of Low-FODMAP Diet in Pediatric Gastroenterology.

    Journal of pediatric gastroenterology and nutrition·2022
    Same author

    Private practice dentists' views of oral health injustice.

    Community dental health·2021
    Same author

    A Systematic Review and Meta-analysis of Paediatric Inflammatory Bowel Disease Incidence and Prevalence Across Europe.

    Journal of Crohn's & colitis·2020
    Same author

    First Measurement of Inclusive Muon Neutrino Charged Current Differential Cross Sections on Argon at E_{ν}∼0.8  GeV with the MicroBooNE Detector.

    Physical review letters·2019

    Area of Science:

    • Microbiology
    • Bacterial Physiology
    • Molecular Biology

    Background:

    • Catabolite repression is a regulatory mechanism controlling the expression of genes involved in carbohydrate metabolism.
    • Cyclic adenosine monophosphate (cAMP) is a crucial second messenger involved in various cellular processes, including gene regulation.
    • Streptomycin dependence in bacteria can alter metabolic regulation.

    Purpose of the Study:

    • To investigate the relationship between streptomycin dependence, catabolite repression, and cyclic AMP levels in Escherichia coli.
    • To determine if relaxed catabolite repression in streptomycin-dependent E. coli is associated with altered intracellular cAMP concentrations.

    Main Methods:

    • Utilizing streptomycin-dependent strains of Escherichia coli B and K-12.

    Related Experiment Videos

  • Culturing bacteria in glucose-salts medium to induce specific metabolic states.
  • Measuring intracellular cyclic AMP concentrations using established biochemical assays.

    • Main Results:

    • Streptomycin-dependent E. coli cultures grown in glucose-salts medium displayed relaxed catabolite repression.
    • These cultures also exhibited significantly elevated concentrations of cyclic AMP compared to control groups.
    • The findings indicate a correlation between relaxed catabolite repression and increased cAMP levels in these bacterial strains.

    Conclusions:

    • Relaxed catabolite repression in streptomycin-dependent Escherichia coli is associated with elevated intracellular cyclic AMP.
    • These results highlight the intricate interplay between nutrient sensing, metabolic control, and second messenger signaling in bacterial physiology.
    • Further research can explore the precise molecular mechanisms underlying this observed phenomenon.