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A Femtoliter Droplet Array for Massively Parallel Protein Synthesis from Single DNA Molecules
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Microfluidic Reactor Array Device for Massively Parallel In-situ Synthesis of Oligonucleotides.

Onnop Srivannavit1, Mayurachat Gulari, Zhishan Hua

  • 1Department of Chemical Engineering, University of Michigan, Ann Arbor, MI 48109, USA.

Sensors and Actuators. B, Chemical
|February 18, 2010
PubMed
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We developed a microfluidic reactor array for synthesizing oligonucleotides (oDNA) in parallel. This device enables efficient, high-yield oDNA synthesis for genomics and proteomics research.

Area of Science:

  • Biotechnology
  • Microfluidics
  • Synthetic Biology

Background:

  • Oligonucleotide (oDNA) synthesis is crucial for genomics and proteomics.
  • Existing methods face challenges in parallelization and reagent control.
  • Need for advanced platforms for high-throughput oDNA synthesis.

Purpose of the Study:

  • To design and fabricate a microfluidic reactor array device.
  • To enable massively parallel, in-situ synthesis of oligonucleotides (oDNA).
  • To address challenges in reagent diffusion and uniform flow in microreactors.

Main Methods:

  • Fabrication of a glass-silicon microfluidic device with microreactors and microchannels.
  • Implementation of a dynamic isolation mechanism to prevent reagent intermixing.

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Last Updated: Jun 16, 2026

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  • Demonstration using solution-based, light-directed parallel in-situ oDNA synthesis.

    • Main Results:

    • Successful synthesis of long oDNA (up to 120 mers) with a high stepwise yield (98%).
    • Achieved uniform flow and synthesis across thousands of parallel reactors.
    • Characterized microarray quality, including sensitivity, specificity, and accuracy.

    Conclusions:

    • The microfluidic reactor array offers a robust platform for parallel oDNA synthesis.
    • The dynamic isolation mechanism effectively prevents reagent diffusion.
    • This technology holds significant potential for advancing genomics and proteomics research.