Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Oxygen Transport in the Blood

Oxygen Transport in the Blood

Hemoglobin (Hb) is a crucial molecule in the human body, consisting of four polypeptide chains, each bound to an iron-containing heme group. This unique structure enables hemoglobin to bind to oxygen, with each molecule capable of combining with four molecules of oxygen, leading to rapid and reversible oxygen loading. When fully loaded with oxygen, it is called oxyhemoglobin, while hemoglobin that has released oxygen is called reduced hemoglobin or deoxyhemoglobin. As hemoglobin binds oxygen,...

Oxygen Requirements and Growth Patterns

Oxygen Requirements and Growth Patterns

Microorganisms exhibit diverse oxygen requirements and growth patterns driven by their metabolic strategies and environmental adaptations. Oxygen, while essential for many organisms, can also be toxic under certain conditions, shaping how microorganisms grow and survive.Oxygen Requirements of MicroorganismsMicroorganisms are classified based on their ability to use or tolerate oxygen:● Obligate aerobes like Mycobacterium tuberculosis need oxygen for energy production, as it serves as the...

Qualitative Analysis

Qualitative Analysis

For solutions containing mixtures of different cations, the identity of each cation can be determined by qualitative analysis. This technique involves a series of selective precipitations with different chemical reagents, each reaction producing a characteristic precipitate for a specific group of cations. Metal ions within a group are further separated by varying the pH, heating the mixture to redissolve a precipitate, or adding other reagents to form complex ions.
For instance, group IV...

Oxygenic Photosynthesis

Oxygenic Photosynthesis

Oxygenic photosynthesis is a fundamental process in which light energy is harnessed to drive the oxidation of water, leading to the production of molecular oxygen (O₂), adenosine triphosphate (ATP), and nicotinamide adenine dinucleotide phosphate (NADPH). This process is essential for sustaining aerobic life on Earth and is primarily carried out by cyanobacteria, algae, and plants. The core of oxygenic photosynthesis lies in the thylakoid membranes, where chlorophyll pigments facilitate light...

Radical Autoxidation

Radical Autoxidation

The oxidation of an organic compound in the presence of air or oxygen is called autoxidation. For example, cumene reacts with oxygen to form hydroperoxide. Autoxidation involves initiation, propagation, and termination steps. Many organic compounds are susceptible to autoxidation—especially ethers in the presence of oxygen, which form hydroperoxides. Even though this reaction is slow, old ether bottles contain small amounts of peroxide, which leads to laboratory explosions during ether...

Atomic Emission Spectroscopy: Interference

Atomic Emission Spectroscopy: Interference

In atomic emission spectroscopy (AES), high-temperature atomizers excite a broad range of elements and molecules that generate complex emissions from sources such as oxides, hydroxides, and flame combustion products in the flame or plasma. Several strategies can be employed to minimize spectral interferences caused by overlapping emission lines or bands. These include increasing instrument resolution, choosing alternative emission lines, optimally placing the detector in low-background regions,...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Hydrolytic and enzymatic degradation of linear segmented polyurethane block copolymers studied by ToF-SIMS and atomic force microscopy.

Biointerphases·2025

Same author

ToF-SIMS analysis of ultrathin films and their fragmentation patterns.

Journal of vacuum science & technology. A, Vacuum, surfaces, and films : an official journal of the American Vacuum Society·2024

Same author

Quantitative evaluation of perfluorinated alkanethiol molecular order on gold surfaces.

Biointerphases·2023

Same author

Back to the basics of time-of-flight secondary ion mass spectrometry data analysis of bio-related samples. II. Data processing and display.

Biointerphases·2023

Same author

Back to the basics of time-of-flight secondary ion mass spectrometry of bio-related samples. I. Instrumentation and data collection.

Biointerphases·2023

Same author

"Chip-on-a-Transwell" Devices for User-Friendly Control of the Microenvironment of Cultured Cells.

ACS applied bio materials·2022

Search research articles

Related Experiment Video

Updated: Jun 16, 2026

Synthesis and Calibration of Phosphorescent Nanoprobes for Oxygen Imaging in Biological Systems

Synthesis and Calibration of Phosphorescent Nanoprobes for Oxygen Imaging in Biological Systems

Published on: March 3, 2010

Amine Terminated SAMs: Investigating Why Oxygen is Present in these Films.

J E Baio¹, T Weidner, J Brison

¹National ESCA and Surface Analysis Center for Biomedical Problems, University of Washington, Departments of Bioengineering and Chemical Engineering, Box 351750, Seattle, WA 98195.

Journal of Electron Spectroscopy and Related Phenomena

|February 18, 2010

Summary

This summary is machine-generated.

This study found that tightly bound water, not oxidation of the amine-terminated alkanethiols, is the main source of unexpected oxygen in self-assembled monolayers (SAMs). Minimizing water improves SAM quality for positively charged surfaces.

More Related Videos

Anaerobic Protein Purification and Kinetic Analysis via Oxygen Electrode for Studying DesB Dioxygenase Activity and Inhibition

Anaerobic Protein Purification and Kinetic Analysis via Oxygen Electrode for Studying DesB Dioxygenase Activity and Inhibition

Published on: October 3, 2018

Oxygen-Independent Assays to Measure Mitochondrial Function in Mammals

Oxygen-Independent Assays to Measure Mitochondrial Function in Mammals

Published on: May 19, 2023

Related Experiment Videos

Last Updated: Jun 16, 2026

Synthesis and Calibration of Phosphorescent Nanoprobes for Oxygen Imaging in Biological Systems

Synthesis and Calibration of Phosphorescent Nanoprobes for Oxygen Imaging in Biological Systems

Published on: March 3, 2010

Anaerobic Protein Purification and Kinetic Analysis via Oxygen Electrode for Studying DesB Dioxygenase Activity and Inhibition

Anaerobic Protein Purification and Kinetic Analysis via Oxygen Electrode for Studying DesB Dioxygenase Activity and Inhibition

Published on: October 3, 2018

Oxygen-Independent Assays to Measure Mitochondrial Function in Mammals

Oxygen-Independent Assays to Measure Mitochondrial Function in Mammals

Published on: May 19, 2023

Area of Science:

Surface Science
Materials Chemistry
Nanotechnology

Background:

Self-assembled monolayers (SAMs) from amine-terminated alkanethiols are common model positively charged surfaces.
Previous studies reported unexpected oxygen-containing species in these SAMs, questioning their surface purity.
Understanding the source of oxygen is crucial for reliable surface characterization and application.

Purpose of the Study:

To identify the source of oxygen species detected in amine-terminated SAMs on gold.
To minimize the presence of these oxygen species for improved surface quality.

Main Methods:

Surface characterization using X-ray photoelectron spectroscopy (XPS).
Time-of-flight secondary ion mass spectroscopy (ToF-SIMS) for high-sensitivity analysis.
Sum frequency generation (SFG) and near edge X-ray absorption fine structure (NEXAFS) spectroscopy for structural and chemical insights.

Main Results:

Achieved significantly lower oxygen concentrations (2.4 ± 0.4 atomic %) compared to previous studies.
XPS, ToF-SIMS, and SFG collectively indicated minimal oxidation of the thiol or amine groups.
Identified tightly bound water as the primary source of detected oxygen, confirmed by SFG spectral features.

Conclusions:

The majority of detected oxygen in amine-terminated SAMs originates from adsorbed water, not inherent oxidation.
This multi-technique investigation provides a clearer understanding of amine-terminated SAM composition.
Minimizing water adsorbates is key to preparing purer, well-defined positively charged surfaces.