Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Cytochalasin B potentiates epinephrine's outflow facility-increasing effect.

J C Robinson1, P L Kaufman

  • 1Department of Ophthalmology, University of Wisconsin School of Medicine, Madison 53792.

Investigative Ophthalmology & Visual Science
|April 1, 1991
PubMed
Summary

Cytochalasin B (CB) and epinephrine (EPI) increase aqueous humor outflow facility in monkeys. Combining subthreshold doses of CB and EPI significantly enhances this effect, suggesting a shared mechanism involving actin filament disruption.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

ArcR modulates biofilm formation in the dental plaque colonizer Streptococcus gordonii.

Molecular oral microbiology·2017
Same author

Weight loss and improved metabolic outcomes amongst rural African American women in the Deep South: six-month outcomes from a community-based randomized trial.

Journal of internal medicine·2017
Same author

Are cholesterol levels being checked and managed appropriately in UK primary care type 2 diabetes?

International journal of clinical practice·2015
Same author

Value and payment for oncology in the United States.

Annales pharmaceutiques francaises·2013
Same author

Accommodative function in rhesus monkeys: effects of aging and calorie restriction.

Age (Dordrecht, Netherlands)·2013
Same author

Surgical Aspects of Typhoid Fever.

Journal of the National Medical Association·2010

Area of Science:

  • Ophthalmology
  • Pharmacology
  • Cell Biology

Background:

  • Understanding the regulation of aqueous humor outflow facility is crucial for managing glaucoma.
  • The trabecular meshwork and Schlemm's canal play key roles in regulating intraocular pressure.
  • Previous research suggests actin filaments in these structures influence outflow facility.

Purpose of the Study:

  • To investigate the combined effects of cytochalasin B (CB) and epinephrine (EPI) on outflow facility in cynomolgus monkeys.
  • To explore the potential synergistic mechanisms by which CB and EPI modulate aqueous humor dynamics.

Main Methods:

  • Constant pressure perfusion technique was used to measure total outflow facility in cynomolgus monkey eyes.
  • Eyes were treated with intracameral cytochalasin B (CB) or vehicle, followed by epinephrine (EPI) or vehicle.

Related Experiment Videos

  • Outflow facility was determined at baseline and after drug administration.

    • Main Results:

    • Subthreshold doses of CB and EPI, ineffective alone, significantly increased outflow facility when administered concurrently.
    • Concurrent subthreshold CB with maximal EPI yielded greater facility increases than maximal EPI alone.
    • Maximal CB demonstrated a greater facility increase than maximal EPI; combined maximal doses showed additive effects.

    Conclusions:

    • CB and EPI exhibit synergistic effects in increasing aqueous humor outflow facility.
    • The findings suggest that both CB and EPI may act by disrupting actin filaments in trabecular meshwork and Schlemm's canal endothelial cells.
    • Epinephrine's mechanism of action may involve altering endothelial cell shape through actin filament disruption, similar to CB.